CRISPR/CAS9-mediated Editing for Pollen-specific <i>OsIPA</i> Genein Rice

YANG Shao-hua; CHEN Hui-mei; ZHOU Shu-fen

doi:10.19303/j.issn.1008-0384.2017.011.001

Volume 32 Issue 11

Jan. 2018

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YANG Shao-hua, CHEN Hui-mei, ZHOU Shu-fen. CRISPR/CAS9-mediated Editing for Pollen-specific OsIPA Genein Rice[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1173-1177. doi: 10.19303/j.issn.1008-0384.2017.011.001

Citation:

YANG Shao-hua, CHEN Hui-mei, ZHOU Shu-fen. CRISPR/CAS9-mediated Editing for Pollen-specific OsIPA Genein Rice[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1173-1177. doi: 10.19303/j.issn.1008-0384.2017.011.001

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CRISPR/CAS9-mediated Editing for Pollen-specific OsIPA Genein Rice

doi: 10.19303/j.issn.1008-0384.2017.011.001

Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture/Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2017-10-11
Rev Recd Date: 2017-11-03
Publish Date: 2017-11-28

Abstract

Abstract

Male gamete development is an essential step in rice reproduction process, and male sterility is crucial for the utilization of rice heterosis. Since the anther or pollen specific genes in rice might play a pivotal role in the male gamete development, the functions of OsIPA were of great interest to the scientists. This study applied CRISPR/CAS9 to generate an OsIPA mutant for an investigation. Two sites in the 1^st exon of OsIPA were selected as the targets. After sequencing, a total of 8 different types of mutant, including deletion, substitution and single nucleotide insertion, were found in 24T₀ transgenic plants.
- rice,
- CRISPR/Cas9,
- gene editing,
- OsIPA

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References(24)

References

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