Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian

CHEN Ru-jing; ZHOU Lun-jiang; WU Xue-min; CHE Yong-liang; WANG Chen-yan; WANG Long-bai; HUANG Xiao-feng; YAN Shan; LIU Yu-tao; WEI Hong

doi:10.19303/j.issn.1008-0384.2017.01.002

Volume 32 Issue 1

Mar. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(1): 7-11

CHEN Ru-jing, ZHOU Lun-jiang, WU Xue-min, CHE Yong-liang, WANG Chen-yan, WANG Long-bai, HUANG Xiao-feng, YAN Shan, LIU Yu-tao, WEI Hong. Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 7-11. doi: 10.19303/j.issn.1008-0384.2017.01.002

Citation:

CHEN Ru-jing, ZHOU Lun-jiang, WU Xue-min, CHE Yong-liang, WANG Chen-yan, WANG Long-bai, HUANG Xiao-feng, YAN Shan, LIU Yu-tao, WEI Hong. Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 7-11. doi: 10.19303/j.issn.1008-0384.2017.01.002

Citation:

CHEN Ru-jing, ZHOU Lun-jiang, WU Xue-min, CHE Yong-liang, WANG Chen-yan, WANG Long-bai, HUANG Xiao-feng, YAN Shan, LIU Yu-tao, WEI Hong. Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 7-11. doi: 10.19303/j.issn.1008-0384.2017.01.002

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Cloning and Sequencing of gB Gene from Porcine Cytomegalovirus Isolated in Fujian

doi: 10.19303/j.issn.1008-0384.2017.01.002

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

Received Date: 2016-07-25
Rev Recd Date: 2016-10-13
Publish Date: 2017-01-01

Abstract

Abstract

Based upon the characterization of gB gene retrieved from GenBank, specific primers were selected to amplify the gB gene of porcine cytomegalovirus (PCMV) FJ01 isolated in Fujian. The target PCR fragments were then cloned and sequenced. The gB gene was found to have 2 580 nucleotides, encoding 859 amino acids. Its homologies on nucleotide and amino acid sequences with the data at GenBank were greater than 98.3% and 97.9%, respectively. In addition, nearly half of the PCMV isolates had a same ACA nucleotide deletion in their gB genes that correlated to the phylogenetic evolution of the isolates, except ZZ strain.
- porcine cytomegalovirus,
- gB gene,
- clone,
- sequence analysis

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References(16)

References

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