Antibiotic-resistance Related to Proteins Associated with Fatty Acid Biosynthesis in <i>Aeromonas hydrophila</i>

HU Wen-jie; ZHANG Xiang-yu; WU Qian; LIN Mei-gui; HUANG Xiao-fang; LIN Xiang-min

doi:10.19303/j.issn.1008-0384.2017.01.001

Volume 32 Issue 1

Mar. 2017

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HU Wen-jie, ZHANG Xiang-yu, WU Qian, LIN Mei-gui, HUANG Xiao-fang, LIN Xiang-min. Antibiotic-resistance Related to Proteins Associated with Fatty Acid Biosynthesis in Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 1-6. doi: 10.19303/j.issn.1008-0384.2017.01.001

Citation:

HU Wen-jie, ZHANG Xiang-yu, WU Qian, LIN Mei-gui, HUANG Xiao-fang, LIN Xiang-min. Antibiotic-resistance Related to Proteins Associated with Fatty Acid Biosynthesis in Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2017, 32(1): 1-6. doi: 10.19303/j.issn.1008-0384.2017.01.001

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Antibiotic-resistance Related to Proteins Associated with Fatty Acid Biosynthesis in Aeromonas hydrophila

doi: 10.19303/j.issn.1008-0384.2017.01.001

HU Wen-jie^{1, 2},
ZHANG Xiang-yu^{1, 2},
WU Qian^{1, 2},
LIN Mei-gui^{1, 2},
HUANG Xiao-fang^{1, 2},
LIN Xiang-min^{1, 2
,
,}

1.
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2016-08-30
Rev Recd Date: 2016-10-20
Publish Date: 2017-01-01

Abstract

Abstract

Widely exists in water and soil, Aeromonas hydrophila is a commonpathogen for human, livestock and fish. It was previously observed in our lab that the expression of the proteins associated with the fatty acid biosynthesis in A. hydrophilarose under the stress of chlortetracycline. But the specific properties and functions were not understood. Therefore, this study was designed to clone and express those potentially important proteins, including acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (AccA), acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (AccD), 3-oxoacyl-[acyl-carrier-protein] synthase Ⅰ (FabB), and malonyl coA-acyl carrier protein transacylase (FabD) for further investigation. By comparing the survival rates of high-expression strains of A. hydrophila under chlortetracycline stress, the drug-resistance relating to the proteinsin the fatty acid biosynthesispathway was analyzed. The results showed that the high-expression proteins, AccD, FabB and FabD, could indeed significantly increase the survival rate of the pathogen under chlortetracycline stress, while AccA did so only during the initial stage. Consequently, a critical role of these proteins involving the bacterial drug-resistance was clearly demonstrated. With the finding, further study to unveil the mechanism of antibiotic-resistance of the pathogen is in order.
- Aeromonas hydrophila,
- fatty acid biosynthesis,
- resistance

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References(23)

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