Cloning and Tissue Expressions of Sucrose Phosphate Synthase Gene in Cassava

HUANG Tang-wei; LUO Xing-lu; SHAN Zhong-ying; ZHU Yan-mei

doi:10.19303/j.issn.1008-0384.2016.12.004

Volume 31 Issue 12

Feb. 2017

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HUANG Tang-wei, LUO Xing-lu, SHAN Zhong-ying, ZHU Yan-mei. Cloning and Tissue Expressions of Sucrose Phosphate Synthase Gene in Cassava[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1273-1279. doi: 10.19303/j.issn.1008-0384.2016.12.004

Citation:

HUANG Tang-wei, LUO Xing-lu, SHAN Zhong-ying, ZHU Yan-mei. Cloning and Tissue Expressions of Sucrose Phosphate Synthase Gene in Cassava[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1273-1279. doi: 10.19303/j.issn.1008-0384.2016.12.004

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Cloning and Tissue Expressions of Sucrose Phosphate Synthase Gene in Cassava

doi: 10.19303/j.issn.1008-0384.2016.12.004

1.
Agricultural College of Guangxi University, Nanning, Guangxi 530005, China
2.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, Guangxi 530004, China

Received Date: 2016-08-22
Rev Recd Date: 2016-10-31
Publish Date: 2016-12-28

Abstract

Abstract

Sucrose phosphate synthase (SPS) gene from cassava RS01 was cloned to study its biological functions. The conserved fragment of the gene was first obtained by homologous cloning, and its full length cDNA by rapid amplification of cDNA ends (RACE). The characteristics, gene sequence and other biological information retrieved were analyzed. Expressions of the genes from various tissues of the plants at different times were compared by RT-qPCR. The results showed that the cDNA of SPS gene had a full length of 3, 857 bp, and an ORF of 3, 228 bp encoding a protein of 1, 076 amino acids. It was subsequently named MeSPS with a GenBank number of KX822780. Its homologous alignments with the nucleotide and protein databases were highly homologs with those of Jatropha carcas L., Ricinus communis L., and Litchi chinensis Sonn. at 89%, 89%, and 87%, respectively. Its amino acid sequence was 77%-92% in similarity with the SPS genes of other plants. The qRT-PCR analysis showed that MeSPS gene was highly expressed during seeding, and, was more expressed in the leaves than the stems or roots on a plant in a same growth period.
- cassava,
- sucrose phosphate synthase,
- gene cloning,
- expression analysis

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References(29)

References

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