Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its <i>UL</i>2 and <i>TK</i> Genes

LIU Rong-chang; HUANG Yu; LU Rong-hui; FU Qiu-ling; JIANG Bin; WAN Chun-he; FU Guang-hua; CHENG Long-fei; SHI Shao-hua; CHEN Hong-mei

doi:10.19303/j.issn.1008-0384.2016.12.001

Volume 31 Issue 12

Feb. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(12): 1257-1261

LIU Rong-chang, HUANG Yu, LU Rong-hui, FU Qiu-ling, JIANG Bin, WAN Chun-he, FU Guang-hua, CHENG Long-fei, SHI Shao-hua, CHEN Hong-mei. Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1257-1261. doi: 10.19303/j.issn.1008-0384.2016.12.001

Citation:

LIU Rong-chang, HUANG Yu, LU Rong-hui, FU Qiu-ling, JIANG Bin, WAN Chun-he, FU Guang-hua, CHENG Long-fei, SHI Shao-hua, CHEN Hong-mei. Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1257-1261. doi: 10.19303/j.issn.1008-0384.2016.12.001

Citation:

LIU Rong-chang, HUANG Yu, LU Rong-hui, FU Qiu-ling, JIANG Bin, WAN Chun-he, FU Guang-hua, CHENG Long-fei, SHI Shao-hua, CHEN Hong-mei. Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1257-1261. doi: 10.19303/j.issn.1008-0384.2016.12.001

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Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes

doi: 10.19303/j.issn.1008-0384.2016.12.001

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Center/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian 350013, China
2.
College of Animal Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2016-08-15
Rev Recd Date: 2016-10-26
Publish Date: 2016-12-28

Abstract

Abstract

In March 2015, massive deaths happened to the M18 Duck Farm and an egg-laying duck farm in Fuzhou. It was suspected of an outbreak of duck plaque. Samples of liver and esophagus from the dead birds were collected for pathogenic identification using PCR and virus isolation. Two strains with virulent characteristics were thus identified. Subsequently, a sequence analysis on their UL2 genes further confirmed the classification. In addition, the TK gene sequences of the two isolated virus strains showed a nucleotide homology of 98.7% with those of the common virulent strain as well as the vaccine strain.
- virulent duck plague virus,
- isolation and identification,
- UL2 gene,
- TK gene,
- sequence analysis

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References(15)

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