Screening Lysophosphatidic-acid-producing <i>Bacillus</i> Using HPLC and Quadrupole Time-of-flight Mass Spectrometry

ZHENG Xue-fang; CHEN Zheng; LIU Bo; ZHU Yu-jing; SHI Huai

doi:10.19303/j.issn.1008-0384.2016.11.021

Volume 31 Issue 11

Jan. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 30(11): 1252-1256

ZHENG Xue-fang, CHEN Zheng, LIU Bo, ZHU Yu-jing, SHI Huai. Screening Lysophosphatidic-acid-producing Bacillus Using HPLC and Quadrupole Time-of-flight Mass Spectrometry[J]. Fujian Journal of Agricultural Sciences, 2016, 30(11): 1252-1256. doi: 10.19303/j.issn.1008-0384.2016.11.021

Citation:

ZHENG Xue-fang, CHEN Zheng, LIU Bo, ZHU Yu-jing, SHI Huai. Screening Lysophosphatidic-acid-producing Bacillus Using HPLC and Quadrupole Time-of-flight Mass Spectrometry[J]. Fujian Journal of Agricultural Sciences, 2016, 30(11): 1252-1256. doi: 10.19303/j.issn.1008-0384.2016.11.021

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Screening Lysophosphatidic-acid-producing Bacillus Using HPLC and Quadrupole Time-of-flight Mass Spectrometry

doi: 10.19303/j.issn.1008-0384.2016.11.021

Agricultural Bio-Resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2016-05-23
Rev Recd Date: 2016-09-08
Publish Date: 2016-11-01

Abstract

Abstract

Lysophosphatidic acid (LPA) is an important extracellular signal transmitter and intracellular secondary messenger. Metabolites in the supernatants of liquid culture media for 34 strains of Bacillus were determined by using HPLC and quadrupole time-of-flight mass spectrometry. It was found that the only B. subtilis strain that produced LPA was FJAT-8784. The obtained LPA showed a matching score of 96.73 according to the Metlin database. It had a molecular weight of 438.2758, and a mass to charge ratio of 439.2819. Consequently, the chemical formula for the isolated LPA was assumed to be C₂₁H₄₃O₇P. In the supernatant, the total metabolites contained approximately 1.32% LPA.
- Bacillus,
- lysophosphatidic acid,
- HPLC/quadrupole time-of-flight mass spectrometry

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References(16)

References

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