Sequence Analysis on gB Gene from Pseudorabies Virus

CHEN Ru-jing; ZHOU Lun-jiang; WU Xue-min; HUANG Xiao-feng; CHE Yong-liang; YAN Shan; WANG Chen-yan; WANG Long-bai; LIU Yu-tao; WEI Hong

doi:10.19303/j.issn.1008-0384.2016.11.001

Volume 31 Issue 11

Jan. 2017

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Fujian Journal of Agricultural Sciences > 2016 > 30(11): 1139-1144. > DOI: 10.19303/j.issn.1008-0384.2016.11.001

CHEN Ru-jing, ZHOU Lun-jiang, WU Xue-min, HUANG Xiao-feng, CHE Yong-liang, YAN Shan, WANG Chen-yan, WANG Long-bai, LIU Yu-tao, WEI Hong. Sequence Analysis on gB Gene from Pseudorabies Virus[J]. Fujian Journal of Agricultural Sciences, 2016, 30(11): 1139-1144. DOI: 10.19303/j.issn.1008-0384.2016.11.001

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Sequence Analysis on gB Gene from Pseudorabies Virus

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

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Received Date: March 31, 2016
Revised Date: May 15, 2016

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Abstract

Abstract

Since 2011, variant pseudorabies (PR) had occurred to the Bartha-k61 vaccinated pigs on farms in most provinces in China. The traditional Bartha-k61 vaccine could not prevent those pigs from contracting the viral disease. As a result, substantial financial losses had incurred to the porcine industry. A previous study indicated that there existed distinctive variations between the gE gene of the typical PR virus (PRV) and that of the variant PRV. For comparison, the sequence of the gB gene of the typical PRV was downloaded from GenBank. It was found that at Positions 395,453,562, and 739 they showed different characteristics, and their nucleotide homology was 98.1%-100% and amino acids 96.1%-100%. A phylogenetic analysis suggested that the viruses were from two different branches and differed significantly depending upon their geographic origins. In China, these viruses had different sub-branches. And, the variant PRV shared a same sub-branch in origin with the newly identified Nyctereutes procyonoides and Yorkshire Terrier PR. The information obtained would be valuable for studying biological functions of gB as well as origin-tracing on variant PRV.
- Pseudorabies virus,
- gB gene,
- sequence analysis

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