Identification and Pathogenicity Assay of Pathogen of Blight Disease on Walnut Trees in Longnan

WANG Han; WANG Rang-jun; TIAN Feng-ming; ZHAO Shu-ling; HE Jiu-jun; LI Zhi-hu; WANG Yi-feng; ZHUO Ping-qing; WANG Ming-xia

doi:10.19303/j.issn.1008-0384.2016.10.016

Volume 31 Issue 10

Dec. 2016

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(10): 1086-1090

WANG Han, WANG Rang-jun, TIAN Feng-ming, ZHAO Shu-ling, HE Jiu-jun, LI Zhi-hu, WANG Yi-feng, ZHUO Ping-qing, WANG Ming-xia. Identification and Pathogenicity Assay of Pathogen of Blight Disease on Walnut Trees in Longnan[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1086-1090. doi: 10.19303/j.issn.1008-0384.2016.10.016

Citation:

WANG Han, WANG Rang-jun, TIAN Feng-ming, ZHAO Shu-ling, HE Jiu-jun, LI Zhi-hu, WANG Yi-feng, ZHUO Ping-qing, WANG Ming-xia. Identification and Pathogenicity Assay of Pathogen of Blight Disease on Walnut Trees in Longnan[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1086-1090. doi: 10.19303/j.issn.1008-0384.2016.10.016

Citation:

WANG Han, WANG Rang-jun, TIAN Feng-ming, ZHAO Shu-ling, HE Jiu-jun, LI Zhi-hu, WANG Yi-feng, ZHUO Ping-qing, WANG Ming-xia. Identification and Pathogenicity Assay of Pathogen of Blight Disease on Walnut Trees in Longnan[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1086-1090. doi: 10.19303/j.issn.1008-0384.2016.10.016

PDF( 1777 KB)

Identification and Pathogenicity Assay of Pathogen of Blight Disease on Walnut Trees in Longnan

doi: 10.19303/j.issn.1008-0384.2016.10.016

1.
College of Agriculture and Forestry, Longnan Teacher's College, Longnan, Gansu 742500, China
2.
Walnut Service Center of Science and Technology of Chengxian County, Longnan, Gansu 742500, China

Received Date: 2016-05-13
Rev Recd Date: 2016-07-19
Publish Date: 2016-10-01

Abstract

Abstract

A pathogen, HT-1, was isolated from the walnut trees that exhibited the typical symptoms of the blight disease. Tests were performed to confirm the pathogenicity and taxonomy of the isolated bacteria. The bacterium was also morphologically identified along with confirmation by a 16S rDNA sequencing analysis to be Pantoea agglomerans. It was found that the open wounds on the tree leaves was the major gateway for the infection. However, in a short term, wounds on the stems did not seem to affect the occurrence of the disease, nor did the roots appear to be an entry for the pathogenic invasion to the plants.
- walnut,
- pathogenic bacteria,
- Pantoea agglomerans,
- pathogenicity

FullText(HTML)

References(15)

References

[1]	ROMERO-SUAREZ S, JORDAN B, HEINEMANN J A. Isolation and characterization of bacteriophages infecting Xanthomonas arboricola pv.juglandis, the causal agent of walnut blight disease[J]. World J Microbiol Biotechnol, 2012, 28(5):1917-1927. doi: 10.1007/s11274-011-0992-z
[2]	DYE D W, BRADBURY J F, GOTO M, et al. International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and patho-types[J]. Review of Plant Pathology, 1980, 59(1):153-168. https://www.cabdirect.org/cabdirect/abstract/19801362818
[3]	BELISARIO A, MACCARONI M, CORAZZA L. Occurrence and etiology of brown apical necrosis on Persan (English) walnut fruit[J]. Plant Disease, 2002, 6(1):599-602. https://www.researchgate.net/publication/249304145_Occurrence_and_Etiology_of_Brown_Apical_Necrosis_on_Persian_English_Walnut_Fruit
[4]	CESBRON S, POTHIER J, GIRONDE S, et al. Development of multilocus variable-number tandem repeat analysis (MLVA) for Xanthomonas arboricola pathovars[J]. J Microbiol Methods, 2014, 100(2):84-90. http://d.scholar.cnki.net/detail/SJESTEMP_U/SJES14032300044051
[5]	方中达.植病研究方法[M].北京:中国农业出版社, 1998:193-194.
[6]	曲文文, 杨克强, 刘会香, 等.山东省核桃主要病害及其综合防治[J].植物保护, 2011, 37(2):136-140. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH201102030.htm
[7]	张永强, 朱惠英, 冯强.核桃黑斑病病原研究与防治试验初报[J].甘肃林业科技, 2004, 29(2):41-42. http://www.cnki.com.cn/Article/CJFDTOTAL-GSLK200402013.htm
[8]	陈善义, 陶万强, 王合, 等.北京地区核桃黑斑病病原菌的分离、致病性测定和16S rDNA序列分析[J].果树学报, 2011, 28(3):469-473. http://www.cnki.com.cn/Article/CJFDTOTAL-GSKK201103019.htm
[9]	严玉宁, 何红, 叶艺俊, 等.香蕉叶鞘腐败病病原鉴定[J].植物病理学报, 2011, 41(2):124-130. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBL201102004.htm
[10]	洪永聪, 胡方平, 黄晓南.成团泛菌对稻谷的致病性[J].福建农林大学学报, 2001, 31(1):32-36. http://www.cnki.com.cn/Article/CJFDTOTAL-FJND200201006.htm
[11]	曲文文.山东省核桃病害病原鉴定[D].泰安:山东农业大学, 2011. http://cdmd.cnki.com.cn/Article/CDMD-10434-1011097996.htm
[12]	NUNES C, USALL J, TEIXIDO N, et al. Post-harvest biological control by Pantoea agglomerans (CPA-2) on Golden Delicious apples[J]. J Appl Microbiol, 2002, 92(2):247-255. doi: 10.1046/j.1365-2672.2002.01524.x
[13]	沈德龙, 冯永君, 宋未.成团肠杆菌的生物功能多样性及其分类最新进展[J].微生物学杂志, 2002, 22(1):40-42. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWX200201015.htm
[14]	黄海东, 李晓雁, 杨红澎, 等.内生菌XJ01的分离鉴定及其对黄瓜褐斑病菌的拮抗作用[J].华北农学报, 2012, 27(4):158-162. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNB201204031.htm
[15]	张晓宇, 张永杰, 张则君, 等.拮抗细菌XM2的鉴定及其对梨采后黑斑病的生防作用[J].微生物学报, 2014, 54(6):649-655. http://www.cnki.com.cn/Article/CJFDTOTAL-WSXB201406008.htm