Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers

CHEN Zhi-dan; WANG An-li; SUN Wei-jiang

doi:10.19303/j.issn.1008-0384.2016.10.011

Volume 31 Issue 10

Dec. 2016

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(10): 1059-1064

CHEN Zhi-dan, WANG An-li, SUN Wei-jiang. Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1059-1064. doi: 10.19303/j.issn.1008-0384.2016.10.011

Citation:

CHEN Zhi-dan, WANG An-li, SUN Wei-jiang. Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1059-1064. doi: 10.19303/j.issn.1008-0384.2016.10.011

Citation:

PDF( 1230 KB)

Genetic Diversity of Tieguanyin and Huangdan Half-sib Tea Cultivars Determined by Using ISSR Markers

doi: 10.19303/j.issn.1008-0384.2016.10.011

CHEN Zhi-dan^{1, 2, 3
,},
WANG An-li³,
SUN Wei-jiang^{1, 2, 3
,
,}

1.
Anxi Tea College, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pest, Fuzhou, Fujian 350002, China
3.
Fujian Provincial Technology Development Base of Tea Industry, Anxi, Fujian 362406, China

Received Date: 2016-07-26
Rev Recd Date: 2016-09-23
Publish Date: 2016-10-28

Abstract

Abstract

Two tea cultivars, Tieguanyin and huangdan, are the core parents used in oolong tea breeding. Many premium oolong tea cultivars have been bred from them. Using ISSR molecular markers, 9 primers with high polymorphism and clearly distinguishable ISSR amplified bands were selected for the amplification of 24 tea varieties (or lines) as well as the determination of the genetic diversity and relationship among the cultivars. A total of 73 bands was generated with 15 ISSR primers. Of which, 47 were polymorphic at a rate of 64.4%, averaging 5.2 bands amplified per primer. An analysis on the genetic similarity of the 24 tea cultivars yielded coefficients ranging from 0.644 to 0.889, with an average of 0.727. It suggested that the genetic bases of the germplasms were limited, and their genetic relationships close. The results would provide valuable information for tea breeding in the future.
- ISSR molecular markers,
- half-sib tea cultivars of Tieguanyin,
- half-sib tea cultivars of Huangdan,
- genetic diversity

FullText(HTML)

References(25)

References

[1]	叶乃兴.我国茶树育种的骨干亲本及其系谱分析[J].中国茶叶, 2008, 4:11-13. http://www.cnki.com.cn/Article/CJFDTOTAL-CAYA200804010.htm
[2]	杨美寅, 李文东, 董方平, 等.湿地松半同胞家系净光合速率日变异规律研究[J].中国农学通报, 2008, 24(8):171-176. http://www.cnki.com.cn/Article/CJFDTOTAL-ZNTB200808037.htm
[3]	韩创举, 杨培华, 刘永红, 等.油松半胞系家系苗期生长性状遗传分析[J].西北农林科技大学学报:自然科学版, 2008, 36(5):124-134. http://www.cnki.com.cn/Article/CJFDTOTAL-XBNY200805022.htm
[4]	莫亚文.刺槐优树半同胞子代测定研究[J].甘肃林业科技, 2007, 32(3):29-32. http://www.cnki.com.cn/Article/CJFDTOTAL-GSLK200703007.htm
[5]	孙明高, 赵玉涛, 宋绪忠, 等.银杏半同胞家系综合评价与苗期选择的研究[J].山东农业大学学报:自然科学版, 2001, 32(4):518-524. http://www.cnki.com.cn/Article/CJFDTOTAL-SCHO200104023.htm
[6]	余继忠.福鼎大白茶半同胞系和云南大叶茶半同胞系遗传多样性和亲缘关系研究[D].北京:中国农业科学院, 2010. http://cdmd.cnki.com.cn/Article/CDMD-82101-2010171383.htm
[7]	余继忠, 杨亚军, 黄海涛, 等.利用ISSR标记分析福云(半)同胞系茶树品种(系)的遗传多样性和亲缘关系[J].基因组学与应用生物学, 2009, 28(2):281-288. http://www.cnki.com.cn/Article/CJFDTOTAL-GXNB200902017.htm
[8]	陈志辉, 单睿阳, 林郑和, 等.利用SSR标记分析福建省选育福云半同胞系茶树品种遗传多样性[J].茶叶学报, 2015, 56(4):238-243. http://www.cnki.com.cn/Article/CJFDTOTAL-CYKJ201504008.htm
[9]	段云裳, 姜燕华, 王丽鸳, 等.我国茶树主要骨干亲本及其衍生品种(系)的SSR分析[J].植物遗传资源学报, 2011, 12(4):533-538. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWYC201104009.htm
[10]	王让剑, 杨军, 孔祥瑞, 等.福建15个茶树品种SSR遗传差异分析与指纹图谱建立[J].福建农业学报, 2014, 29(10):970-975. http://www.cnki.com.cn/Article/CJFDTOTAL-FJNX201410008.htm
[11]	ZIETKIEWICZ E, RAFALSKI A, and LABUDA D.Genome fingerprintingby simple sequence repeat (SSR)-anchored polymerase chain reaction, amplification, Genomics, 1994, 20(2):176-183. doi: 10.1006/geno.1994.1151
[12]	REDDY M P, SARLA N, SIDDIQ E A.Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding[J].Euphytica, 2002, 128(1):9-17. doi: 10.1023/A:1020691618797
[13]	姚明哲, 黄海涛, 余继忠, 等. ISSR在茶树品种分子鉴别和亲缘关系研究中的适用性分析[J].茶叶科学, 2005, 25(2):153-157. http://www.cnki.com.cn/Article/CJFDTOTAL-CYKK200502013.htm
[14]	林郑和, 陈荣冰, 陈常颂, 等.ISSR分子标记在茶树遗传关系分析中的初步应用[J].茶叶科学, 2007, 27(1):45-50. http://www.cnki.com.cn/Article/CJFDTOTAL-CYKK200701007.htm
[15]	唐玉海, 郭春芳, 张木清, 等.ISSR标记在茶树品种遗传多态性研究中的应用[J].福建农林大学学报:自然科学版, 2007, 36(1):51-55. http://www.cnki.com.cn/Article/CJFDTOTAL-FJND200701011.htm
[16]	黄建安, 黄意欢, 罗军武, 等.茶树基因组DNA的高效提取方法[J].湖南农业大学学报:自然科学版, 2003, 29(5):402-407. http://www.cnki.com.cn/Article/CJFDTOTAL-HNND200305011.htm
[17]	詹梓金, 李宗恒, 徐飚, 等.保护铁观音原种保持优异品质--论铁观音品种提纯复壮[J].茶叶科学技术, 2006, (2):20. http://www.cnki.com.cn/Article/CJFDTOTAL-CYKJ200602007.htm
[18]	詹梓金, 林锻炼, 陈磊.安溪铁观音品种复壮内涵与防止退化对策[J].福建茶叶, 2009, (1):12-13. http://www.cnki.com.cn/Article/CJFDTOTAL-FJCA200901007.htm
[19]	景润春, 何予卿, 黄青阳, 等.水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析[J].中国农业科学, 2000, 33(2):1-9. http://www.cnki.com.cn/Article/CJFDTOTAL-ZNYK200002001.htm
[20]	KAUNDUN S S, ZHYVOLOUP A, PARK Y G.Evaluation of the genetic diversity among elite tea (Camellia sinensis var.sinensis) accessions using RAPD markers[J].Euphytica, 2000, 115:7-16. doi: 10.1023/A:1003939120048
[21]	PARK Y G, SHIV S K.Genetic variation on wild tea populations in Korea[M]//Liang Y R, Liu Z S, Park Y G, et al.Proceedings of the International Symposium on Molecular Biology and TeaBreeding.Hangzhou:Zhejiang University, 2000:1-18.
[22]	WACHIRA F N, WAUGH R, HACKETT C A, et al.Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers[J].Genome, 1995, 38:201-210. doi: 10.1139/g95-025
[23]	MIRSHRA R K, SEN-MANDI S.Genetic diversity estimates for Darjeeling tea clones based on amplified fragment length polymorphism markers[J].J Tea Sci, 2004, 24(2):86-92. http://en.cnki.com.cn/Article_en/CJFDTOTAL-CYKK200402002.htm
[24]	刘本英, 孙雪梅, 李友勇, 等.基于EST-SSR标记的云南无性系茶树良种遗传多样性分析及指纹图谱构建[J].茶叶科学, 2012, 32(3):261-268. http://www.cnki.com.cn/Article/CJFDTOTAL-CYKK201203012.htm
[25]	杨亚军, 梁月荣.中国无性系茶树品种志[M].上海:上海科学技术出版社, 2014:111, 146.