Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian

ZHU Hai-xia; ZENG Liang-ming; CHEN Lei; ZHANG Yuan-kui

doi:10.19303/j.issn.1008-0384.2016.10.004

Volume 31 Issue 10

Dec. 2016

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ZHU Hai-xia, ZENG Liang-ming, CHEN Lei, ZHANG Yuan-kui. Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1024-1027. doi: 10.19303/j.issn.1008-0384.2016.10.004

Citation:

ZHU Hai-xia, ZENG Liang-ming, CHEN Lei, ZHANG Yuan-kui. Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1024-1027. doi: 10.19303/j.issn.1008-0384.2016.10.004

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Sequence Analysis on ORF3 Gene of Porcine Epidemic Diarrhea Virus in Fujian

doi: 10.19303/j.issn.1008-0384.2016.10.004

Fuzhou Dabeinong Biotechnology Co., Ltd., Fuzhou, Fujian 350014, China

Received Date: 2016-06-23
Rev Recd Date: 2016-08-12
Publish Date: 2016-10-28

Abstract

Abstract

To study the ORF3 gene of porcine epidemic diarrhea virus (PEDV) in Fujian, samples were collected from regions in the province during 2014-2015. Eight strains were amplified for sequence alignment and phylogenetic analysis. Genetic evolution analysis showed that the strains were of wild variety and close to those found domestically and in the neighboring countries in recent years with up to 100% homology on the nucleotide and deduced amino acids. But, they were distantly related to the classical strain of CV777, with a nucleotide homology of 96.0%-96.9% and amino acid homology of 92.5%-94.7%. It appeared that genetic alternations had occurred to the PEDV strain currently isolated from the samples collected in the province.
- porcine epidemic diarrhea,
- ORF3 gene,
- genetic variation

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References(10)

References

[1]	PENSAERT M B, DE BOUCK P.A new coronavirus-like particle associated with diarrhea in swine[J]. Arch virol, 1978, 58(3):243-247. doi: 10.1007/BF01317606
[2]	王隆柏, 吴学敏, 车勇良, 等.猪流行性腹泻病毒ORF3和M基因的克隆与序列分析[J].中国动物传染病学报, 2012, 20(3):67-72. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSJB201203015.htm
[3]	孙贤刚, 唐蕊涵, 蔡雨函, 等.2011-2012年四川省猪流行性腹泻病毒的遗传变异分析[J].中国兽医学报, 2015, 35(1):16-21. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSYX201501004.htm
[4]	董丽娜.猪流行性腹泻病毒COE基因乳酸菌表达载体的构建与表达研究[D].吉林:吉林农业大学, 2008, 1-5. http://cdmd.cnki.com.cn/Article/CDMD-10193-2009162981.htm
[5]	WANG K, LU W, CHEN J, et al.PEDV ORF3 encodes an ion channel protein and regulates virus production[J].Febs Letters, 2012, 586(4):384-391. doi: 10.1016/j.febslet.2012.01.005
[6]	PARK S J, MOON H J, LUO Y, et al.Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea virus[J].Virus Genes, 2008, 36:95-104. doi: 10.1007/s11262-007-0164-2
[7]	高君恺, 刘浩飞, 杨倩.猪流行性腹泻病毒的研究进展[J].南京农业大学学报, 2014, 37(1):1-5. http://www.cnki.com.cn/Article/CJFDTOTAL-NJNY201401001.htm
[8]	刘琪, 冯晓声, 周如月, 等.鉴别猪流行性腹泻疫苗毒株与野毒株巢式PCR检测方法的建立及初步应用[J].广东畜牧兽医科技, 2015, 40(2):36-38. http://www.cnki.com.cn/Article/CJFDTOTAL-GDXS201502015.htm
[9]	CHEN J F, SUN D B, WANG C B, et al. Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China. Virus Genes, 2008, 36:355-364. doi: 10.1007/s11262-007-0196-7
[10]	CHEN J F, WANG C B, SHI H Y, et al. Molecular epidemiology of porcine epidemic diarrhea virus in China[J]. Arch Virology, 2010, 155:1471-1476. doi: 10.1007/s00705-010-0720-2