Construction and Certification of Recombinant Adenovirus Vector for σC Gene in Muscovy Duck Reovirus

LIN Feng-qiang; CHENG Xiao-xia; WANG Shao; ZHU Xiao-li; WANG Jin-xiang; CHEN Shi-long; CHEN Shao-ying

doi:10.19303/j.issn.1008-0384.2016.10.002

Volume 31 Issue 10

Dec. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(10): 1015-1019

LIN Feng-qiang, CHENG Xiao-xia, WANG Shao, ZHU Xiao-li, WANG Jin-xiang, CHEN Shi-long, CHEN Shao-ying. Construction and Certification of Recombinant Adenovirus Vector for σC Gene in Muscovy Duck Reovirus[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1015-1019. doi: 10.19303/j.issn.1008-0384.2016.10.002

Citation:

LIN Feng-qiang, CHENG Xiao-xia, WANG Shao, ZHU Xiao-li, WANG Jin-xiang, CHEN Shi-long, CHEN Shao-ying. Construction and Certification of Recombinant Adenovirus Vector for σC Gene in Muscovy Duck Reovirus[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1015-1019. doi: 10.19303/j.issn.1008-0384.2016.10.002

Citation:

LIN Feng-qiang, CHENG Xiao-xia, WANG Shao, ZHU Xiao-li, WANG Jin-xiang, CHEN Shi-long, CHEN Shao-ying. Construction and Certification of Recombinant Adenovirus Vector for σC Gene in Muscovy Duck Reovirus[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1015-1019. doi: 10.19303/j.issn.1008-0384.2016.10.002

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Construction and Certification of Recombinant Adenovirus Vector for σC Gene in Muscovy Duck Reovirus

doi: 10.19303/j.issn.1008-0384.2016.10.002

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

Received Date: 2016-03-29
Rev Recd Date: 2016-07-06
Publish Date: 2016-10-28

Abstract

Abstract

The σC gene of muscovy duck reovirus (MDRV) was amplified by RT-PCR and cloned into pShuttle-CMV plasmid after the amplicon was digested by XhoI+Hind III enzyme. Sequencing analysis confirmed the gene to be σC. Subsequently, the recombinant plasmid pShuttle-CMV-σC and the adenovirus backbone vector, pAdEasy-1, were co-transfected into 293 cells to construct a recombinant adenovirus. The obtained recombinant adenovirus pAd-σC was positively identified by PCR and RT-PCR. The information obtained could be used for genetic engineering a vaccine against MDRV.
- muscovy duck reovirus,
- σC gene,
- recombinant adenovirus vector

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References(13)

References

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