Formation and Determination of Saquinavir from Liquid Fermentation of <i>Bacillus aryabhattai</i> FJAT-14220

ZHU Yu-jing; LIU Bo; ZHENG Mei-xia; CHEN Zheng

doi:10.19303/j.issn.1008-0384.2016.09.016

Volume 31 Issue 9

Nov. 2016

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ZHU Yu-jing, LIU Bo, ZHENG Mei-xia, CHEN Zheng. Formation and Determination of Saquinavir from Liquid Fermentation of Bacillus aryabhattai FJAT-14220[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 981-985. doi: 10.19303/j.issn.1008-0384.2016.09.016

Citation:

ZHU Yu-jing, LIU Bo, ZHENG Mei-xia, CHEN Zheng. Formation and Determination of Saquinavir from Liquid Fermentation of Bacillus aryabhattai FJAT-14220[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 981-985. doi: 10.19303/j.issn.1008-0384.2016.09.016

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Formation and Determination of Saquinavir from Liquid Fermentation of Bacillus aryabhattai FJAT-14220

doi: 10.19303/j.issn.1008-0384.2016.09.016

Aricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2015-11-11
Rev Recd Date: 2016-04-29
Publish Date: 2016-09-28

Abstract

Abstract

Extracellular metabolites of Bacillus aryabhattai FJAT-14220 during fermentation were analyzed with liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry. Saquinavir in the fermentation broth was targeted for the study. Molecular feature extraction and database retrieval were applied to access the information on the metabolites of B. aryabhattai. A total of 883 compounds were detected with 148 of them identified. The matching score of saquinavir was 93.08 with a relative concentration of 1.73%. Its retention time was 2.7191 min; and, accurate mass, 670.3837. The results could be the foundation for future development and utilization of saquinavir by B. aryabhattai fermentation.
- liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry,
- Bacillus aryabhattai,
- extracellular metabolites,
- saquinavir

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References(18)

References

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