Prokaryotic Expression and Polyclonal Antibody for Ferric Uptake Regulator Prepared from <i>Vibrio vulnificus</i> FJ03-X2

LI Su-yi; Zhang Li-juan; CHEN Hua; KE Ling; CHEN Xu; LIN Chen-tao

doi:10.19303/j.issn.1008-0384.2016.09.003

Volume 31 Issue 9

Nov. 2016

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LI Su-yi, Zhang Li-juan, CHEN Hua, KE Ling, CHEN Xu, LIN Chen-tao. Prokaryotic Expression and Polyclonal Antibody for Ferric Uptake Regulator Prepared from Vibrio vulnificus FJ03-X2[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 912-916. doi: 10.19303/j.issn.1008-0384.2016.09.003

Citation:

LI Su-yi, Zhang Li-juan, CHEN Hua, KE Ling, CHEN Xu, LIN Chen-tao. Prokaryotic Expression and Polyclonal Antibody for Ferric Uptake Regulator Prepared from Vibrio vulnificus FJ03-X2[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 912-916. doi: 10.19303/j.issn.1008-0384.2016.09.003

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Prokaryotic Expression and Polyclonal Antibody for Ferric Uptake Regulator Prepared from Vibrio vulnificus FJ03-X2

doi: 10.19303/j.issn.1008-0384.2016.09.003

Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2016-06-11
Rev Recd Date: 2016-07-18
Publish Date: 2016-09-28

Abstract

Abstract

Ferric uptake regulator (fur) gene from Vibrio vulnificus FJ03-X2 was cloned, and inserted into the prokaryotic expression vector, pET32a. Coding sequence of the gene contained 450 bps, encoding 149 amino acids. Its sequencing showed a greater than 98% homology with that of the fur gene from GenBank. Subsequently, the plasmid, pET32a-FUR, was transformed into E. coli BL21 to express the recombinant protein, rFUR. The soluble rFUR was then subject to the Ni-NTA His Binding affinity purification. The purified rFUR was injected into SD rats to produce polyclonal antibody. The obtained highly specific antibodies of FUR was confirmed by ELISA assay.
- Vibrio vulnificus,
- ferric uptake regulator,
- cloning,
- prokaryotic expression,
- polyclonal antibody

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References(17)

References

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