Development of Indirect ELISA for Detecting Novel Duck Reovirus Using σB and σC Proteins as Coating Antigens

WANG Jin-xiang; CHENG Xiao-xia; CHEN Shao-ying; CHEN Shi-long; LIN Feng-qiang; WANG Shao; ZHU Xiao-li; XIAO Shi-feng

doi:10.19303/j.issn.1008-0384.2016.09.001

Volume 31 Issue 9

Nov. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(9): 903-907

WANG Jin-xiang, CHENG Xiao-xia, CHEN Shao-ying, CHEN Shi-long, LIN Feng-qiang, WANG Shao, ZHU Xiao-li, XIAO Shi-feng. Development of Indirect ELISA for Detecting Novel Duck Reovirus Using σB and σC Proteins as Coating Antigens[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 903-907. doi: 10.19303/j.issn.1008-0384.2016.09.001

Citation:

WANG Jin-xiang, CHENG Xiao-xia, CHEN Shao-ying, CHEN Shi-long, LIN Feng-qiang, WANG Shao, ZHU Xiao-li, XIAO Shi-feng. Development of Indirect ELISA for Detecting Novel Duck Reovirus Using σB and σC Proteins as Coating Antigens[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 903-907. doi: 10.19303/j.issn.1008-0384.2016.09.001

Citation:

WANG Jin-xiang, CHENG Xiao-xia, CHEN Shao-ying, CHEN Shi-long, LIN Feng-qiang, WANG Shao, ZHU Xiao-li, XIAO Shi-feng. Development of Indirect ELISA for Detecting Novel Duck Reovirus Using σB and σC Proteins as Coating Antigens[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 903-907. doi: 10.19303/j.issn.1008-0384.2016.09.001

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Development of Indirect ELISA for Detecting Novel Duck Reovirus Using σB and σC Proteins as Coating Antigens

doi: 10.19303/j.issn.1008-0384.2016.09.001

WANG Jin-xiang^{1, 2},
CHENG Xiao-xia^{1, 2},
CHEN Shao-ying^{1, 2
,
,},
CHEN Shi-long^{1, 2},
LIN Feng-qiang^{1, 2},
WANG Shao^{1, 2},
ZHU Xiao-li^{1, 2},
XIAO Shi-feng^{1, 2}

1.
Institute of Animal Husbandry and veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350013, China
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

Received Date: 2016-03-04
Rev Recd Date: 2016-05-12
Publish Date: 2016-09-28

Abstract

Abstract

An indirect ELISA was developed using the purified recombinant novel duck reovirus(NDRV)σB and σC proteins as coating antigens. The optimized conditions for the methodology were determined to include:concentrations of σB at 12.5 μg·mL^-1 and σC at 6.25 μg·mL^-1,a blocking buffer of 15% FBS, serum samples being diluted 80 times, goat anti-duck HRP-IgG being diluted 400 times,and substrate incubation at 37℃ for 5 min. The assay was found to be specific without any cross reaction with antibodies of MDRV, DHV, MPV, or MD-GPV. The coincidence rate between the indirect ELISA coated with the recombinant σB and σC proteins and that coated with NDRVwas 92.5%. It appeared that the newly developed ELISA exhibited satisfactory sensitivity and specificity on measurements,and could be an alternative means for detecting anti-NDRV antibodies.
- novel duck reovirus,
- prokaryotic expression,
- σB protein,
- σC protein,
- indirect ELISA

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References(13)

References

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