Embryo Culture for Orchid Hybrid Crossedbetween Beallara Marfitch and Oncidium Sweet Sugar

Embryos of the orchidhdyridcrossed between Beallara Marfitch Howards Dream and Oncidium Sweet Sugar Million Dollar were usedas explants in a cultivation experimentation to study the effects of various growth regulators and organic additives on its germination, protocorm differentiation, and rooting, from the formations of embryos and protocorms to the development and transplantation of the plants. Aculture medium consisting of Hyponex, 6-BA 0.5 mg·L^-1, NAA 0.1 mg·L^-1, CM 150 g·L^-1, AC 1.0 g·L^-1, and sugar 20 g·L^-1 provided an optimal relative embryo germination rate of 155.6% for the hybrid after 90-100 d of cultivation. In addition, the medium inhibited the protocorm proliferation, and thus, encouraged its differentiation subsequently. For the protocorm differentiation, a medium containing Hyponex, 6-BA 0.1 mg·L^-1, NAA 0.1 mg·L^-1, mashed potato 150 g·L^-1, AC 1.0 g·L^-1, and sugar 25 g·L^-1 rendered asignificantly higher rate of 87.8% thanother media tested. The addition of mashed potatoes in the medium significantly increased the differentiation ratepromoting the seedling growth. Vigorously growing plantlets were, then, transferred to a medium containing Hyponex, NAA 0.2 mg·L^-1, mashed potato 150 g·L^-1, AC 1.5 g·L^-1, and sugar 25 g·L^-1 for a complete and well-developed rooting on the healthy and well-developed plantlets with 40 d of cultivation.After an initial adaptation period, the survival rate of the transplanted seedling sreached 91.7% on peat moss.