Prokaryotic Expression of 3 GT Gene from <i>Narcissus tazetta</i> var. Chinensis

WANG Wei-Ying; LI Hai-Ming; DAI Yi-Min; ZOU Hui; WU Shui-Jin; LI Yue-Sen; LIN Jiang-Bo

doi:10.19303/j.issn.1008-0384.2016.08.005

Volume 31 Issue 8

Oct. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(8): 816-819

WANG Wei-Ying, LI Hai-Ming, DAI Yi-Min, ZOU Hui, WU Shui-Jin, LI Yue-Sen, LIN Jiang-Bo. Prokaryotic Expression of 3 GT Gene from Narcissus tazetta var. Chinensis[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 816-819. doi: 10.19303/j.issn.1008-0384.2016.08.005

Citation:

WANG Wei-Ying, LI Hai-Ming, DAI Yi-Min, ZOU Hui, WU Shui-Jin, LI Yue-Sen, LIN Jiang-Bo. Prokaryotic Expression of 3 GT Gene from Narcissus tazetta var. Chinensis[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 816-819. doi: 10.19303/j.issn.1008-0384.2016.08.005

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Prokaryotic Expression of 3 GT Gene from Narcissus tazetta var. Chinensis

doi: 10.19303/j.issn.1008-0384.2016.08.005

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China

Received Date: 2016-05-16
Rev Recd Date: 2016-05-30
Publish Date: 2016-08-01

Abstract

Abstract

Flavonoid 3-O-glucosyltransferase(Nt3GT) gene of Narcissus tazetta var. Chinensis was cloned into the vector, pET29a, to construct prokaryotic expression recombinant plasmid, pET29a-NT3GT.The recombinant plasmid was transferred to E.coli BL21(DE3) to be induced by IPTG. Subsequently, the expression of the target protein was verified by SDS-PAGE. Then, pET29a-Nt3GT was successfully constructed, which induced the 55 kDa recombinant protein of Nt3GT in the prokaryotic expression system. Nt3GT was cloned, and its vector was constructed and induced to be expressed successfully by IPTG in BL21 providing a basis for theantiserum preparation and functional analysis in the future.
- Narcissus tazetta var. Chinensis,
- 3GT,
- prokaryotic expression

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References(14)

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