Response Surface Optimization of Enzymatic Extraction Loach Polysaccharides

DANG Xiao-yan; WANG Yin; SU Yong-chang; LIU Shu-ji; WU Cheng-ye; CHEN Li-jiao

doi:10.19303/j.issn.1008-0384.2016.07.017

Volume 31 Issue 7

Oct. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(7): 752-757

DANG Xiao-yan, WANG Yin, SU Yong-chang, LIU Shu-ji, WU Cheng-ye, CHEN Li-jiao. Response Surface Optimization of Enzymatic Extraction Loach Polysaccharides[J]. Fujian Journal of Agricultural Sciences, 2016, 31(7): 752-757. doi: 10.19303/j.issn.1008-0384.2016.07.017

Citation:

DANG Xiao-yan, WANG Yin, SU Yong-chang, LIU Shu-ji, WU Cheng-ye, CHEN Li-jiao. Response Surface Optimization of Enzymatic Extraction Loach Polysaccharides[J]. Fujian Journal of Agricultural Sciences, 2016, 31(7): 752-757. doi: 10.19303/j.issn.1008-0384.2016.07.017

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Response Surface Optimization of Enzymatic Extraction Loach Polysaccharides

doi: 10.19303/j.issn.1008-0384.2016.07.017

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen, Fujian 361013, China

Received Date: 2016-05-10
Rev Recd Date: 2016-06-20
Publish Date: 2016-07-01

Abstract

Abstract

In order to optimize the extraction process of polysaccharides loach ultrasonic enzyme samples in 100 W ultrasound first ultrasonic instrument 20 min, and then in the enzymatic test based on a single factor, select the hydrolysis temperature, reaction time and dosage neutral protease from variable polysaccharide extraction rate response, the central composite design, study their interactions on polysaccharide extraction rate. The response surface analysis methods, simulated prediction model quadratic polynomial regression equation and determine the best conditions of the Loach Polysaccharide Extraction Process:enzymatic hydrolysis temperature of 50℃, enzymatic hydrolysis time 6 h, neutral protease amount of 5% in under this condition, the polysaccharide was 5.37%.
- loach,
- polysaccharides,
- ultrasonic enzymatic,
- response surface

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References(17)

References

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