Screening and Identifying Endophytic Fungi from Single-clump Tea Plants

CAI Li; CHEN He-xia; LI Sheng-zhi; FU Li

doi:10.19303/j.issn.1008-0384.2016.04.011

Volume 31 Issue 4

Jun. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(4): 389-394

CAI Li, CHEN He-xia, LI Sheng-zhi, FU Li. Screening and Identifying Endophytic Fungi from Single-clump Tea Plants[J]. Fujian Journal of Agricultural Sciences, 2016, 31(4): 389-394. doi: 10.19303/j.issn.1008-0384.2016.04.011

Citation:

CAI Li, CHEN He-xia, LI Sheng-zhi, FU Li. Screening and Identifying Endophytic Fungi from Single-clump Tea Plants[J]. Fujian Journal of Agricultural Sciences, 2016, 31(4): 389-394. doi: 10.19303/j.issn.1008-0384.2016.04.011

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Screening and Identifying Endophytic Fungi from Single-clump Tea Plants

doi: 10.19303/j.issn.1008-0384.2016.04.011

CAI Li¹,
CHEN He-xia¹,
LI Sheng-zhi²,
FU Li^{3
,
,}

1.
College of Food Science and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
2.
Department of Food Science and Biotechnology, Chung Hsing Univeresity, Taizhong, Taiwan 402, China;
3.
College of Life Sciences and Food Technology, Hanshan Normal University, Chaozhou, Guangdong 521041, China

Received Date: 2016-01-27
Rev Recd Date: 2016-04-05
Publish Date: 2016-04-28

Abstract

Abstract

Endophytic fungi antagonistic against the selected bacteria and phytopathogens were first screened from the microbial samples collected from the single-clump tea plants using the double-plate and the flat-stand methods. Then, they were identified by their morphologies and 18SrDNA sequences. Three strains of the fungi, i.e., F15GEN, F15Ye, and LB35JING, were isolated. The diameter of the inhibition zone of F15GEN against Staphylococcus aureus was 13.36 mm, and that of F15YE against Bacillus subtilis 14.92 mm. In addition, LB35JING and F15GEN showed inhibition activities against Rhizoctonia solani and Valsa ambiens Pers, F15YE against Sphaerulina juglandis, and LB35JING against Fusarium. The 18S rDNA sequence of F15GEN identified it as Penicillium decumbens, while those of F15YE and LB35JING linked them to Penicillium sp.
- single-clump tea,
- endophytic fungi,
- phytopathogens,
- inhibition activity,
- identification

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References(4)

References

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