Cloning and Sequence Analysis of an Odorant Binding Protein Gene <em>PxylOBP13</em> in the Diamondback Moth, <em>Plutella xylostella</em> (Lepidoptera:Plutellidae)

QIN Jiang-mei; CHENG Xiao-juan; WEI Hui; CAI Li-jun; YOU Min-sheng

doi:10.19303/j.issn.1008-0384.2016.04.009

Volume 31 Issue 4

Jun. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(4): 377-383

QIN Jiang-mei, CHENG Xiao-juan, WEI Hui, CAI Li-jun, YOU Min-sheng. Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP13 in the Diamondback Moth, Plutella xylostella (Lepidoptera:Plutellidae)[J]. Fujian Journal of Agricultural Sciences, 2016, 31(4): 377-383. doi: 10.19303/j.issn.1008-0384.2016.04.009

Citation:

QIN Jiang-mei, CHENG Xiao-juan, WEI Hui, CAI Li-jun, YOU Min-sheng. Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP13 in the Diamondback Moth, Plutella xylostella (Lepidoptera:Plutellidae)[J]. Fujian Journal of Agricultural Sciences, 2016, 31(4): 377-383. doi: 10.19303/j.issn.1008-0384.2016.04.009

Citation:

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Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP13 in the Diamondback Moth, Plutella xylostella (Lepidoptera:Plutellidae)

doi: 10.19303/j.issn.1008-0384.2016.04.009

QIN Jiang-mei^1,2,3,4,
CHENG Xiao-juan^1,2,3,5,
WEI Hui⁶,
CAI Li-jun^{1,2,3,4
,
,},
YOU Min-sheng^{1,2,3,4
,
,}

1.
Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
3.
Key Laboratory of Integrated Pest Management of Fujian and Taiwan, China Ministry of Agriculture, Fuzhou, Fujian 350002, China;
4.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
5.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
6.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2016-01-23
Rev Recd Date: 2016-02-25
Publish Date: 2016-04-28

Abstract

Abstract

The diamondback moth (DBM), Plutella xylostella (L.) is a key insect pest on cruciferous plants all over the world. Olfactory perception is critical for survival and reproduction in insects, and correspondingly provides a new target to control DBM. Hereby, an odorant binding protein (OBP) gene from P.xylostella,PxylOBP13 (GenBank accession number:KT156679) was cloned; the bio-information characteristics were analyzed; and a phylogenetic tree was constructed. The results showed that the full-length of open reading frame of PxylOBP13 is 387 bp and encodes 128 amino acids; PxylOBP13 contains 6 conserved cysteine residues and the conserved domain of odorant binding protein family, and 3 hydrophobic regions which are potential binding sites for hydrophobic odorants. The protein molecular mass was predicted as 14.33 kDa with an isoelectric point of 7.55, but the N-terminal without signal peptide; the similarity of sequence between PxylOBP13 and six lepidopteran insects can reach above 50%. The results can provide theoretical support for further research on the function of OBPs in DBM and development of effective management of DBM based on olfactory mechanism.
- Plutella xylostella,
- odorant binding protein,
- cloning,
- sequence analysis

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References(8)

References

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