Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology

CHE Jian-mei; LIU Bo; MA Gui-mei; LIU Guo-hong; TANG Jian-yang

doi:10.19303/j.issn.1008-0384.2015.11.012

Volume 30 Issue 11

Nov. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(11): 1090-1096

CHE Jian-mei, LIU Bo, MA Gui-mei, LIU Guo-hong, TANG Jian-yang. Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1090-1096. doi: 10.19303/j.issn.1008-0384.2015.11.012

Citation:

CHE Jian-mei, LIU Bo, MA Gui-mei, LIU Guo-hong, TANG Jian-yang. Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1090-1096. doi: 10.19303/j.issn.1008-0384.2015.11.012

Citation:

CHE Jian-mei, LIU Bo, MA Gui-mei, LIU Guo-hong, TANG Jian-yang. Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1090-1096. doi: 10.19303/j.issn.1008-0384.2015.11.012

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Optimization on Ultrasonic Disruption for Extracting Intracellular Metabolites of Brevibacillus brevis FJAT-0809-GLX Using Response Surface Methodology

doi: 10.19303/j.issn.1008-0384.2015.11.012

1. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2. Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2015-10-08
Publish Date: 2015-11-18

Abstract

Abstract

Ultrasound was applied to rupture the cells of Brevibacilllus brevis FJAT-0809-GLX to facilitate extraction of the intracellular metabolites produced by the bacteria.Effect of ultrasonic power,treatment time,and cell/liquid ratio were studied in a single-factor experiment.Optimum conditions of the extraction were determined using a design-expert program and the response surface method.The single-factor experiment showed that,when the ultrasonic treatment time was 30 min,the greatest recovery rate on the intracellular metabolites,i.e.,11mg·g^-1,could be obtained;when 100 W of ultrasound power was applied,the highest extraction rate of9.33mg·g^-1 was achieved;and,when a cell/liquid ratio was 1∶25implemented,the highest yield of 12.22mg·g^-1 could be reached.Then,the process was optimization by using the response surface method to show that the effects were cell/liquid ratio>ultrasonic application time>ultrasonic power,and the optimal conditions for the ultrasonic disruption were:an application of ultrasonic power at 318.68 W,a total treatment time of 10 min,and a cell/liquid ratio of 1∶25.As a result,ayield of the intracellular metabolites from the extraction at 12.987 8mg·g^-1 was achieved.
- ultrasonic disruption,
- Brevibacillus brevis,
- response surface method,
- metabolite extraction

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References(37)

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