Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor

ZHENG Xue-li; LIU Sheng-cai; XIE Li-yang; CHENG Chun-zhen; LAI Zhong-xiong

doi:10.19303/j.issn.1008-0384.2015.11.008

Volume 30 Issue 11

Nov. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(11): 1064-1070

ZHENG Xue-li, LIU Sheng-cai, XIE Li-yang, CHENG Chun-zhen, LAI Zhong-xiong. Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008

Citation:

ZHENG Xue-li, LIU Sheng-cai, XIE Li-yang, CHENG Chun-zhen, LAI Zhong-xiong. Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008

Citation:

ZHENG Xue-li, LIU Sheng-cai, XIE Li-yang, CHENG Chun-zhen, LAI Zhong-xiong. Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1064-1070. doi: 10.19303/j.issn.1008-0384.2015.11.008

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Promoter-cloning,Protein Subcellular Localization,and Expression Analysis for AmaDOPA5-GT Gene of Amaranth tricolor

doi: 10.19303/j.issn.1008-0384.2015.11.008

1. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. Institute of Vegetable Science of Fuzhou, Fuzhou, Fujian 350111, China

Received Date: 2015-09-28
Publish Date: 2015-11-18

Abstract

Abstract

Bioinformatic characteristics and protein subcellular localization of AmaDOPA5-GT gene in Amaranth tricolor were studied.The chromosome walking technique was applied to clone the gene promoter.The protein was found to be located in the cytomembrane.Eight light-responsive elements were identified in the promoter,suggesting that light might be associated with the regulation of the gene expression.The amaranthin content and quantitative real time PCR showed that darkness suppressed both betalain biosynthesis and AmaDOPA5-GT gene expression;red light also suppressed betalain biosynthesis,but significantly enhanced the gene expression;yellow and green light showed no effect on the gene expression,but suppressed the betalain biosynthesis;and,blue light induced both betalain biosynthesis and the gene expression.The results obtained provided a basis for further exploring the function of AmaDOPA5-GT in betalain biosynthesis,as well as a guideline for breeding betalain-rich amarnath.
- amaranth,
- DOPA5-GT,
- promoter,
- light,
- betalain biosynthesis

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References(24)

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