Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report

LI Xiao-lin; NIU Zhi-gang; YUAN Yan; WANG Shan; LI Lie-gang; SHI Hong-cai

doi:10.19303/j.issn.1008-0384.2015.11.002

Volume 30 Issue 11

Nov. 2015

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(11): 1032-1040

LI Xiao-lin, NIU Zhi-gang, YUAN Yan, WANG Shan, LI Lie-gang, SHI Hong-cai. Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1032-1040. doi: 10.19303/j.issn.1008-0384.2015.11.002

Citation:

LI Xiao-lin, NIU Zhi-gang, YUAN Yan, WANG Shan, LI Lie-gang, SHI Hong-cai. Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1032-1040. doi: 10.19303/j.issn.1008-0384.2015.11.002

Citation:

LI Xiao-lin, NIU Zhi-gang, YUAN Yan, WANG Shan, LI Lie-gang, SHI Hong-cai. Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1032-1040. doi: 10.19303/j.issn.1008-0384.2015.11.002

PDF( 1227 KB)

Correlation Between Prolactin SNPs and Milk Producing of Chinese Hu Sheep——A Preliminary Report

doi: 10.19303/j.issn.1008-0384.2015.11.002

1. Key Laboratory of Genetics, Breeding and Reproduction of Grass Feeding Livestock, Ministry of Agriculture/The Key Lab of Animal Biotechnological of Xinjiang, Biotechnological Center, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China;
2. Xinjiang Huang Cattle Livestock Development CO. Ltd., Urumqi, Xinjiang 830000, China

Received Date: 2015-08-27
Publish Date: 2015-11-18

Abstract

Abstract

This study aimed to find the genetic markers that control the milk-producing traits of Hu sheep for breeding purpose.Molecular genetic polymorphism of the prolactin gene in the sheep populations was studied.More than 41 parturition ewes were used by separating the ewes from the lambs 20 hours on the 4th day after giving birth,and manually lactated to collect milk from them.On the following day,the milk collection took place at 7am,1∶30pm,and 7pm every day for 8weeks.Each milk sample was analyzed for its chemical composition.After the entire gene sequence was obtained for organizing and analyzing by SPSS 13.0to locate and identify the SNP loci by MEGA6,followed by the linkage and haplotype analysis.Finally,using the least squares method,the correlation between the polymorphisms and the milk-producing traits was established.The results showed that the average milk content of Hu sheep during 56 days was(0.52±0.07)kg·d^-1,and the fat,protein,sugar,and total solids contents in the milk were(3.83±0.90)%,(5.52±0.63)%,(5.50±0.73)%,and(15.13±1.61)%,respectively.Based on the genetic and the linkage disequilibrium analyses,4 milk-producing traits were found to significantly correlate with the SNP sites in Hu sheep.At the PRL4244 site,the milk producing amount of the GG type in the 8th week of lactation was significantly higher than the TT genotype(P<0.05);at the PR1039 site,the fat content in the milk during mid-term of the AC-type was significantly higher than AA and CC types;at the PRL1228 site,the fat content of the AC-type was significantly higher than AA and CC types in the early stage(P<0.05),while that of the AC type during mid-term was significantly higher than AA and CC types(P<0.01);the total solids of AC in mid-term were significantly higher than those of the CC genotype(P<0.05),and the total solids of AC type in the mid-term was significantly higher than the CC type(P<0.05);at PR2351 sites,the fat contents of the AG type in the early stage and mid-term were significantly higher than AA and GG genotype(P<0.05),while the protein ratio of the AA type in mid-term was significantly higher than the AG and the GG genotype(P<0.01),and the total solids of the AG type in mid-term were significantly higher than those of the AA or the GG genotype(P <0.01);and,at PR4591 sites,the milk fat content of the CC type in the early stage was significantly higher than the TT genotype(P<0.01),while the protein ratio of the CC type in mid-term was significantly higher than those of the TT and TC types(P<0.01),and the total solids was significantly higher than the TT type in the early stage.There were a total of 25 haplotypes detected,the frequency of 7haplotypes were more than 0.02,and the protein content of CCATT haplotype was significantly higher than that of AAATT haloytpe.No significant diffience were existed in the milk ingredient and milk content among other haplotypes.
- Hu sheep,
- PRL polymorphism,
- milk-producing

FullText(HTML)

References(20)

References

[1]	RUSSO V M,CAMERON A W N,DUNSHEA F R,et al.Artificially extending photoperiod improves milk yield in dairy goats and is most effective in late lactation[J].Small Ruminant Research,2013,113:179-186.
[2]	EDYTA M,TOMASZ M,KATARZYNA R,et al.Short-day and melatonin effects on milking parameters,prolactin profiles and growth-hormone secretion in lactating sheep[J].Small Ruminant Research,2013,109:182-187.
[3]	ASIF MAHMOOD,SUMARIA USMAN.A comparative study on the physicochemical parameters of milk samples collected from buffalo,cow,goat and sheep of Gujrat[J].Pakistan Journal of Nutrition,2010,9(12):1196-1197.
[4]	张耀,郭定宗.催乳素结构与功能研究进展[J].动物医学进展,2007,28(5):49-52.
[5]	KELLY P A,BACHELOT A,KEDZIA C,et al.The role of prolactin and growth hormone in mammary gland development[J].Mol Cell Endocrinol,2002,197(2):127-131.
[6]	HARIGAYA T,KOMORI M,WATANABE H,et al.Prolactin gene expression in mouse pancreatic islets.[J]Endocrinol,2002,73(1):29-34.
[7]	HORIGUCHI K,YAGI S,ONO K,et al.Prolactin gene expressioin in mouse spleen helper T cells.[J]Endocrinol,2004,183(3):639-646.
[8]	ARANDA J,RIVERA J C,JEZIORSKI M C,et al.Prolactin are natural inhibitors of angiogenesis in the retina[J].Invest Ophthalmol VisSci,2005,46(8):2947-2953.
[9]	赵秀华,褚明星,刘荣志,等.催乳素基因的研究进展[J].遗传繁育,2007,34(11):28-34.
[10]	J·萨姆布鲁克,D W拉塞尔.分子克隆实验指南:第3版[M].北京:科学出版社,2002,8.
[11]	何凡.人工培育藏猪种群SLA-DRB和POU1F1基因SNP与与生长发育性状的关联分析[D].四川:四川农业大学,2013.
[12]	CRISA A,MARCHITELLI C,PARISET L G,et al.Exploring polymorphisms and effects of candidate genes on milk fat quality in dairy sheep[J].J Dairy Sci,2010,93:3834-3845.
[13]	HADJIPAVLOU G,ORFORD M,TZAMALOUKAS O,et al.Genetic analysis of Chios sheep:Candidate genetic loci and their association with milkTrait[C]//4th International Conference of Quantitative Genetics,2012:257-259.
[14]	UDINA I G,TURKOVA S O,KOSTIUCHENKO M V,et al.G E.Polymorphisn of cattle prolactin gene;microsatellites,PSR-RFLP[J].Genetika Apr,2001,37(4):511-516.
[15]	VERA-LASTRAO,JARA L J,ESPINOZA L R,et al.Prolactin and autoimmunity.Autoimmun[J].Rev,2002,1(6):360-364.
[16]	BRYM P,KAMINSKI S,WOJCIK E,et al.Nucleotide sequence polymorphism within exon 4of the bovine prolactin gene and its associations with milk performance traits[J].J Appl Genet,2005,45(2):179-185.
[17]	SCHRADIN C,ANZENBERGER G.Development of prolactin levels inmarmoset males:From adult son to first-time father[J].Horm B-havior,2004,46(5):670-677.
[18]	李吉涛,杜立新.中国荷斯坦牛催乳素基因型与产奶性状的相关分析[J].山东农业大学学报,2004,35(4):553-555.
[19]	KHATAMI S R,LAZEBNYI O E,MAKSIMENKO V F,et al.Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and black-and-white cattle[J].Genetika,2005,42(2):229-236.
[20]	ELSA GARCIA-GAMEZ,MARTA GARCIA-FERNANDEZ,JOSE ALEJANDRO MORAN,et al.Association analysis between Prolactin SNPs and milk production traits in Spanish Churra sheep:preliminary results[C]//ISAG,2010.