Detection and Sequence Analysis of Potato Spindle Tuber Viroid Found in Fujian

LI Hua-wei; LUO Wen-bin; JI Rong-chang; XU Yong-qing; ZHANG Hong; LI Guo-liang; LIU Zhong-hua; QIU Yong-xiang; QIU Si-xin; TANG Hao

doi:10.19303/j.issn.1008-0384.2015.10.012

Volume 30 Issue 10

Oct. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(10): 984-988

LI Hua-wei, LUO Wen-bin, JI Rong-chang, XU Yong-qing, ZHANG Hong, LI Guo-liang, LIU Zhong-hua, QIU Yong-xiang, QIU Si-xin, TANG Hao. Detection and Sequence Analysis of Potato Spindle Tuber Viroid Found in Fujian[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 984-988. doi: 10.19303/j.issn.1008-0384.2015.10.012

Citation:

LI Hua-wei, LUO Wen-bin, JI Rong-chang, XU Yong-qing, ZHANG Hong, LI Guo-liang, LIU Zhong-hua, QIU Yong-xiang, QIU Si-xin, TANG Hao. Detection and Sequence Analysis of Potato Spindle Tuber Viroid Found in Fujian[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 984-988. doi: 10.19303/j.issn.1008-0384.2015.10.012

Citation:

LI Hua-wei, LUO Wen-bin, JI Rong-chang, XU Yong-qing, ZHANG Hong, LI Guo-liang, LIU Zhong-hua, QIU Yong-xiang, QIU Si-xin, TANG Hao. Detection and Sequence Analysis of Potato Spindle Tuber Viroid Found in Fujian[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 984-988. doi: 10.19303/j.issn.1008-0384.2015.10.012

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Detection and Sequence Analysis of Potato Spindle Tuber Viroid Found in Fujian

doi: 10.19303/j.issn.1008-0384.2015.10.012

1. Crop Institute Research Centre, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2. Root and Tuber Crops Research Centre, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2015-08-08
Publish Date: 2015-10-18

Abstract

Abstract

A new methodology was developed for etection and determination of the molecular variation of the potato spindle tuber viroid found in Fujian.A pair of primers was designed and synthesized based on the STVd gene isolated and amplified from the extracted viral RNA with RT-PCR.The specific fragment of 359 bp was isolated from the infected plants,but none from the healthy tissue samples.The fragment was amplified and subjected to validation by agarose gel electrophoresis.Then,the purified PCR product was cloned,sequenced and Blast searched to be identified as PSTVd isolate KM588065.1.The isolate shared 98% homogeneity with that from NCBI data bank.Comparing the sequence alignments of 3 other strains,the isolate appeared to contain 11 nucleotide polymorphisms with 5in the pathogenicity region.Based on the phylogenetic tree analysis on the DNA sequences of PSTVd from different countries and regions,this virus isolate was determined to be closely related to those from northern China,Europe and America,and only remotely related to that from New Zealand.
- Fujian,
- potato,
- potato spindle tuber viroid,
- RT-PCR,
- detection

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References(20)

References

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