Diversity of Endophytic Fungi in Leaves of Dendrobium candidum

CHEN Ze-bin; LI Bing; WANG Ding-kang; YU Lei; LIU Jia-ni; XU Sheng-guang; JIN Song; REN Zhen

doi:10.19303/j.issn.1008-0384.2015.10.011

Volume 30 Issue 10

Oct. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(10): 978-983

CHEN Ze-bin, LI Bing, WANG Ding-kang, YU Lei, LIU Jia-ni, XU Sheng-guang, JIN Song, REN Zhen. Diversity of Endophytic Fungi in Leaves of Dendrobium candidum[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 978-983. doi: 10.19303/j.issn.1008-0384.2015.10.011

Citation:

CHEN Ze-bin, LI Bing, WANG Ding-kang, YU Lei, LIU Jia-ni, XU Sheng-guang, JIN Song, REN Zhen. Diversity of Endophytic Fungi in Leaves of Dendrobium candidum[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 978-983. doi: 10.19303/j.issn.1008-0384.2015.10.011

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Diversity of Endophytic Fungi in Leaves of Dendrobium candidum

doi: 10.19303/j.issn.1008-0384.2015.10.011

1. Agriculture College, Kunming University, Kunming, Yunnan 650214, China;
2. Engineering Research Center for Characteristics Agriculture of Yunnan Province, Kunming, Yunnan 650214, China;
3. Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan 650223, China;
4. Life Science and Technology Department, Kunming University, Kunming, Yunnan 650214, China

Received Date: 2015-08-15
Publish Date: 2015-10-18

Abstract

Abstract

To determine the diversity of the endophytic fungi grown on Dendrobium candidum,the ITS2 region from the samples was sequenced using the Illumina MiSeq high-through put sequencing method.The number of sequences and operational taxonomic units(OTUs)of each sample were sorted and calculated using Qiime and Mothur software.The species abundance and distribution,alpha diversity index,and time of species abundance of the fungi were determined.The number of effective sequences was found to be 41 361;and the OTUs was 33.The rarefaction functions indicated an adequate sampling,while the OTU number was close to saturation.It was concluded that the endophytic fungi found in D.candidumbelonged to Emericella,and E.nidulans was the dominant species.
- Dendrobium candidum,
- endophytic fungi,
- high-throughput sequencing,
- Emericella nidulans

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References(41)

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