Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011

LI An-qi; LAN Jiang-lin; LIU Bo; HUANG Su-fang; WANG Xiao-ying

doi:10.19303/j.issn.1008-0384.2015.10.008

Volume 30 Issue 10

Oct. 2015

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LI An-qi, LAN Jiang-lin, LIU Bo, HUANG Su-fang, WANG Xiao-ying. Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 958-964. doi: 10.19303/j.issn.1008-0384.2015.10.008

Citation:

LI An-qi, LAN Jiang-lin, LIU Bo, HUANG Su-fang, WANG Xiao-ying. Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 958-964. doi: 10.19303/j.issn.1008-0384.2015.10.008

Citation:

LI An-qi, LAN Jiang-lin, LIU Bo, HUANG Su-fang, WANG Xiao-ying. Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 958-964. doi: 10.19303/j.issn.1008-0384.2015.10.008

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Antibacterial Efficacy and Optimized Protease-producing Capability of Brevibacillus formosus FJAT-10011

doi: 10.19303/j.issn.1008-0384.2015.10.008

1.College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;
2.Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2015-09-09
Publish Date: 2015-10-18

Abstract

Abstract

Antibacterial efficacy andprotease-producing capability of Brevibacillus formosus FJAT-10011 were studied.Screening and optimizing the culture conditions were conducted with a single-factor test and an orthogonal experiment.The optimal medium was determined to contain 1.25% starch,1.25% peptone,0.3% beef extract powder,0.5% NaCl,0.05% MgSO4,and 0.05% K2 HPO4.The optimized fermentation process included the following conditions:an initial pH of 5.5,a temperature at 25℃,the filling of the liquid medium at 30 mL/250 mL,a bacterial inoculum rate at 1%,and a fermentation duration of 24 h.Under these conditions,the protease activity of the supernatant of the liquid medium reached 8.41U·mL-1,which was 141 times greater than that in the beginning of the fermentation.The diameter of the antibacterial zone shown on a petri dish test was 15.71 mm,a 35.31%increase as compared to the non-optimized control.
- protease,
- antibacterial efficacy,
- fermentation optimization,
- single factor,
- orthogonal test

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References(24)

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