Cloning GPX Gene and Bioinformatics of Putian Pear,Pyruscalleryana Decne

Aglutathione peroxidasegene(GPX)was cloned from the leaves of Putian pear,PyruscalleryanaDecne.The RT-PCR and RACE were applied to obtain the complete cDNA sequence for GPX.The full length of the cDNA was approximately 932 bp consisting of an open reading frame of 507 bpwith the 5′-and 3′-untranslated regions of204 bp and 221 bp,respectively.The putative protein had 168 amino acids and was greater than 90% homogenous on the sequence with Maluspumila,Citrus reticulata Banco,Dimocarpuslongan and Litchi chinensis.The gene had been registered in GenBankwith a code of JQ011278.The nucleotide and amino acid sequences of GPX were analyzed by using a bioinformatics software.The results showed that the protein had a molecular weight of 20083.0Da anda theoretical pI 5.61.It was a hydrophilic cytoplasmic protein without transmembrane domain and signal peptide.The cell was mainly located in the cytoplasm at a region most likely to form coiled-coilcontaining26.58% α-spiral,19.26%extending chain and 54.16%irregular curl.And,there were 12 phosphorylation sites on the cell.A3-Dmolecular structure of the enzyme was proposed with relevant analysis.