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YE Wei, LIN Yu-ling, KUANG Yun-bo, JIANG Jin-lan, LI Yong-qing, LEI Fu-gui, LAI Zhong-xiong. Enhancing Effect of Transferred Ferredoxin Proteins in Oncidiumon Disease-resistance of Longan[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9): 850-855. doi: 10.19303/j.issn.1008-0384.2015.09.005
Citation:
YE Wei, LIN Yu-ling, KUANG Yun-bo, JIANG Jin-lan, LI Yong-qing, LEI Fu-gui, LAI Zhong-xiong. Enhancing Effect of Transferred Ferredoxin Proteins in Oncidiumon Disease-resistance of Longan[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9): 850-855. doi: 10.19303/j.issn.1008-0384.2015.09.005
YE Wei, LIN Yu-ling, KUANG Yun-bo, JIANG Jin-lan, LI Yong-qing, LEI Fu-gui, LAI Zhong-xiong. Enhancing Effect of Transferred Ferredoxin Proteins in Oncidiumon Disease-resistance of Longan[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9): 850-855. doi: 10.19303/j.issn.1008-0384.2015.09.005
Citation:
YE Wei, LIN Yu-ling, KUANG Yun-bo, JIANG Jin-lan, LI Yong-qing, LEI Fu-gui, LAI Zhong-xiong. Enhancing Effect of Transferred Ferredoxin Proteins in Oncidiumon Disease-resistance of Longan[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9): 850-855. doi: 10.19303/j.issn.1008-0384.2015.09.005
To study the enhancing effect on the disease-resistance of longan by genetic modification,twononphotosynthetic ferredoxins from the plant were transferred to Oncdiumthrough Agrobacterium tumefaciens strain EHA105 for cultivation on a culture medium containing 5mg·L-1hygromycin.As compared to the plants in the wild,the Fd3 transgenic plants showed a significantly higher resistance to soft rot disease improving the resistance grading from highly sensitive(Di=100)to moderate(Di=27.5).In addition,the survival rate of the transplants also increased significantly from 79.3%to 95.7%.
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