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ZHANG Peng, ZHAO Guang-hui, LIN Jin-xiu, ZHU Jian, ZHUANG Wei-dong. A Preliminary Study on Regeneration of PleurotuseryngiiProtoplast[J]. Fujian Journal of Agricultural Sciences, 2015, 30(8): 784-788. doi: 10.19303/j.issn.1008-0384.2015.08.012
Citation:
ZHANG Peng, ZHAO Guang-hui, LIN Jin-xiu, ZHU Jian, ZHUANG Wei-dong. A Preliminary Study on Regeneration of PleurotuseryngiiProtoplast[J]. Fujian Journal of Agricultural Sciences, 2015, 30(8): 784-788. doi: 10.19303/j.issn.1008-0384.2015.08.012
ZHANG Peng, ZHAO Guang-hui, LIN Jin-xiu, ZHU Jian, ZHUANG Wei-dong. A Preliminary Study on Regeneration of PleurotuseryngiiProtoplast[J]. Fujian Journal of Agricultural Sciences, 2015, 30(8): 784-788. doi: 10.19303/j.issn.1008-0384.2015.08.012
Citation:
ZHANG Peng, ZHAO Guang-hui, LIN Jin-xiu, ZHU Jian, ZHUANG Wei-dong. A Preliminary Study on Regeneration of PleurotuseryngiiProtoplast[J]. Fujian Journal of Agricultural Sciences, 2015, 30(8): 784-788. doi: 10.19303/j.issn.1008-0384.2015.08.012
Effect of regenerating method,osmotic pressure stabilizer,culture medium,age of mushroom,etc.on the regeneration of Pleurotus eryngii protoplast was investigated.The results showed that the protoplasts from the 5-7days old mycelia yielded the most desirable results when cultured in a yeast peptone regeneration medium with up to 0.9% of NaCl as the osmotic stabilizer.Lowered temperature,shortened time and reduced enzyme concentration could help minimize enzymatic hydrolysis in the preparation which would benefit the protoplast regeneration.
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