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LIN Ya-rong, ZHOU Shu-fen, ZHU Yi-wang, SUN Qing-shan, CHEN Hui-mei, WANG Feng. Genome Editing and Its Application for Genetic Improvements of Plants[J]. Fujian Journal of Agricultural Sciences, 2015, 30(5): 522-527. doi: 10.19303/j.issn.1008-0384.2015.05.019
Citation:
LIN Ya-rong, ZHOU Shu-fen, ZHU Yi-wang, SUN Qing-shan, CHEN Hui-mei, WANG Feng. Genome Editing and Its Application for Genetic Improvements of Plants[J]. Fujian Journal of Agricultural Sciences, 2015, 30(5): 522-527. doi: 10.19303/j.issn.1008-0384.2015.05.019
LIN Ya-rong, ZHOU Shu-fen, ZHU Yi-wang, SUN Qing-shan, CHEN Hui-mei, WANG Feng. Genome Editing and Its Application for Genetic Improvements of Plants[J]. Fujian Journal of Agricultural Sciences, 2015, 30(5): 522-527. doi: 10.19303/j.issn.1008-0384.2015.05.019
Citation:
LIN Ya-rong, ZHOU Shu-fen, ZHU Yi-wang, SUN Qing-shan, CHEN Hui-mei, WANG Feng. Genome Editing and Its Application for Genetic Improvements of Plants[J]. Fujian Journal of Agricultural Sciences, 2015, 30(5): 522-527. doi: 10.19303/j.issn.1008-0384.2015.05.019
Technology of precise genome editing based on the transcription activator-like effector nucleases(TALENs)and the type II clustered regularly interpaced short palindromic repeats(CRISPR/Cas9)has been a powerful auxiliary tool for research on genetic functions and improvements of plants.TALENs and CRISPR/Cas9 can induce a double strand break(DSB)between the bound sequence resulting in cellular repairs on DSB through either the homologous recombination(HR)or non-homologous end joining(NHEJ)pathway prior to making modifications on the target genome.This article reviews the information relevant to TALENs and CRISPR/Cas9 based on the structure characteristics,action principle and applications of target modification on plants.Potential applications of the advanced technology were also discussed.
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