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Volume 30 Issue 3
Mar.  2015
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WANG Mei, SHI Jing, TAN De-shui, XU Yu, CUI Rong-zong, JIANG Li-hua. Characterization of β-1,4-D-mannanase from Bacillus subtilis MSJ-5[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 293-297. doi: 10.19303/j.issn.1008-0384.2015.03.017
Citation: WANG Mei, SHI Jing, TAN De-shui, XU Yu, CUI Rong-zong, JIANG Li-hua. Characterization of β-1,4-D-mannanase from Bacillus subtilis MSJ-5[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 293-297. doi: 10.19303/j.issn.1008-0384.2015.03.017

Characterization of β-1,4-D-mannanase from Bacillus subtilis MSJ-5

doi: 10.19303/j.issn.1008-0384.2015.03.017
  • Received Date: 2014-09-03
  • Publish Date: 2015-03-18
  • The characterization ofβ-1,4-D-mannanase producd by MSJ-5,isolated from konjac soil, was investigated.Cultivating in shake-flask at 32℃for 32 h,the enzyme activity reached the highest value and then the mount ofβ-1,4-D-mannanase peaked.In addition,the optimum conditions for the enzymatic activity are pH7.0and50℃,and it is stabilized at pH 5.0-7.0.Furthermore,Konjac mannan was efficiently hydrolyzed by the MSJ-5mannanase and its viscosity was reduced significantly,and the product was mainly a mannooligsaccharide.Our results suggest that MSJ-5has the application potential to the feed enzyme preparation and oligosaccharide industry.
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