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CHEN Han-xin. Establishment of Tissue Culture System for Siraitia grosvenorii[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 253-257. doi: 10.19303/j.issn.1008-0384.2015.03.009
Citation:
CHEN Han-xin. Establishment of Tissue Culture System for Siraitia grosvenorii[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 253-257. doi: 10.19303/j.issn.1008-0384.2015.03.009
CHEN Han-xin. Establishment of Tissue Culture System for Siraitia grosvenorii[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 253-257. doi: 10.19303/j.issn.1008-0384.2015.03.009
Citation:
CHEN Han-xin. Establishment of Tissue Culture System for Siraitia grosvenorii[J]. Fujian Journal of Agricultural Sciences, 2015, 30(3): 253-257. doi: 10.19303/j.issn.1008-0384.2015.03.009
The stem segment of Siraitia grosvenorii was used as the material for establishing of tissue culture system with micropropogation.The results showed that the medium of MS + 6-BA 1.0mg·L-1+ NAA 0.10mg·L-1+sucrose 30g·L-1+agar 6.0g·L-1 was suitable for inducing axillary bud with induction rate of 40.0%.Single nodes cutting and accelerating for axillary buds growth were applied in subculture and optimal medium was 2/3in modified MS + IBA 0.5 mg·L-1+ GA3 0.3 mg·L-1+ sucrose 40g·L-1+ agar 6.0 g·L-1,with proliferation coefficient 4.0.The optimal rooting medium was 1/2in modified MS +IBA 0.5mg·L-1+ sucrose2.0% + AC 0.1% + agar 6.0g·L-1,with rooting rate of 94%.The suitable transplanting medium was peat soil+ pearlite in the ratio of 10∶2,with survival rate for 97.8%.
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