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FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LIN Bing. Development of Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Bulbous Iris[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 172-175. doi: 10.19303/j.issn.1008-0384.2015.02.013
Citation:
FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LIN Bing. Development of Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Bulbous Iris[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 172-175. doi: 10.19303/j.issn.1008-0384.2015.02.013
FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LIN Bing. Development of Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Bulbous Iris[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 172-175. doi: 10.19303/j.issn.1008-0384.2015.02.013
Citation:
FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LIN Bing. Development of Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Bulbous Iris[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 172-175. doi: 10.19303/j.issn.1008-0384.2015.02.013
By using specific primers designed on the basis of conserved sequences of coat protein(CP)gene,a multiplex PCR protocol for the detection of three main viruses(Iris mild mosaic virus,IMMV;Narcissus latent virus,NLV;Bean yellow mosaic virus,BYMV)in Bulbous Iris was developed.Three specific fragments were simultaneously amplified in one PCR reaction system.Their molecular weights were 770 bp,266bp and186 bp respectively.Sequence analysis indicated that the three virus sequences shared at least 98 percent identities with that of other relative viruses.The result showed that the sensitivity of this protocol equaled to be able to detect the three viruses at the level of 10-2 mg Bulbous Iris tissue.
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