Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato

XU Yong-qing; LI Hua-wei; QIU Si-xin; LIU Zhong-hua; QIU Yong-xiang; LUO Wen-bin; JI Rong-chang; TANG Hao; YU Hua

doi:10.19303/j.issn.1008-0384.2014.11.014

Volume 29 Issue 11

Nov. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(11): 1114-1117

XU Yong-qing, LI Hua-wei, QIU Si-xin, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, YU Hua. Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1114-1117. doi: 10.19303/j.issn.1008-0384.2014.11.014

Citation:

XU Yong-qing, LI Hua-wei, QIU Si-xin, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, YU Hua. Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1114-1117. doi: 10.19303/j.issn.1008-0384.2014.11.014

Citation:

XU Yong-qing, LI Hua-wei, QIU Si-xin, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, YU Hua. Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1114-1117. doi: 10.19303/j.issn.1008-0384.2014.11.014

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Development of Dutiplex RT-PCR for the Detection of SPFMV and SPCSV in Sweet Potato

doi: 10.19303/j.issn.1008-0384.2014.11.014

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences;
2. Central Laboratory of Agricultural Sciences of Fujian Academy

Received Date: 2014-07-30
Publish Date: 2014-11-18

Abstract

Abstract

A duplex PCR protocol for the detection of two main viruses in sweet potato was developed based on the coat protein (CP) gene of Sweet potato feathery mottle virus (SPFMV) and the (Hsp70) gene of Sweet potato chlorotic stunt virus (SPCSV) .They were able to amplify unique DNA fragments of 461 bp and 304 bp in a single PCR reaction.The sequence analysis suggst that the two viruses shared at least 93 percent homology with other related viruses.
- sweet potato,
- SPCSV,
- SPFMV,
- duplex RT-PCR

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References(10)

References

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