Effects of Colchicine on Mutagenesis of OncidiumIn Vitro

YE Xiu-xian; HUANG Min-ling; FAN Rong-hui; WU Jian-she; LUO Yuan-hua

doi:10.19303/j.issn.1008-0384.2014.11.008

Volume 29 Issue 11

Nov. 2014

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YE Xiu-xian, HUANG Min-ling, FAN Rong-hui, WU Jian-she, LUO Yuan-hua. Effects of Colchicine on Mutagenesis of OncidiumIn Vitro[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1083-1087. doi: 10.19303/j.issn.1008-0384.2014.11.008

Citation:

YE Xiu-xian, HUANG Min-ling, FAN Rong-hui, WU Jian-she, LUO Yuan-hua. Effects of Colchicine on Mutagenesis of OncidiumIn Vitro[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1083-1087. doi: 10.19303/j.issn.1008-0384.2014.11.008

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Effects of Colchicine on Mutagenesis of OncidiumIn Vitro

doi: 10.19303/j.issn.1008-0384.2014.11.008

YE Xiu-xian^1,2,3,
HUANG Min-ling^1,2,3,
FAN Rong-hui^1,2,3,
WU Jian-she^1,2,3,
LUO Yuan-hua^1,2,3

1. Institute of Crop Sciences,Fujian Academy of Agricultural Science;
2. Flowers Research Center ,Fujian Academy of Agricultural Science;
3. Fujian Engineering Research Center for Characteristic Floriculture

Received Date: 2014-08-04
Publish Date: 2014-11-18

Abstract

Abstract

With colchicine was used as the mutagenic agent, combined with tissue culture techniques, the mutagenesis experiments for bud clump and PLB of Oncidium and the molecular marker technology to detect mutagenesis progeny were studied.Resultsshowed that method of mixed medium supplemented with colchicine was the best way to mutage the Oncidium, and the best condition was 10 dinduction with 500 mg·L-1of colchicine.Plant mutation rate was as high as 26.7%, and there were different mutation forms, such as hairchested, polyphyllous, dwarfed plantlets and widened, thickened leaves, and linear, patterned leaves.Mutant strain went through 5⁶successive transfer culture, and part of them remained properties well.Among them, the variation with golden margin leaves and variation with green margin golden leaves had apparent variation compared with the original species on DNA level by RAPD markers.It suggested a new breeding methods and ideas for Oncidium.
- Oncidium,
- culture in vitro,
- protocorm-like body,
- colchicines,
- chemical mutagenesis

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References(20)

References

[1]	何松林, 十鸟三和子, 王献, 等.不同基本培养基及培养方式对文心兰原球茎增殖的影响[J].华北农学报, 2001, 16 (1) :88-91.
[2]	何松林, 十鸟三和子.基本培养基及凝固剂对文心兰试管苗生长发育的影响[J].北京林业大学学报, 2001, 23 (1) :29-31.
[3]	丁兰, 付庭治.兰花生物工程研究进展[J].西北师范大学学报, 2000, 36 (3) :111-115.
[4]	叶炜, 尚伟, 夏潮水.文心兰育种研究进展[J].三明农业科技, 2008, (3) :17-19.
[5]	邱金.文心兰的杂交育种[J].花卉, 2010, (4) :18-19.
[6]	范成明, 李枝林, 何月秋.兰花组织培养及分子生物学研究进展[J].园艺学报, 2003, 30 (4) :487-491.
[7]	刘玉进, 郑成木.诱变结合植物组织培养在植物育种中的应用[J].上海农业学报, 2004, (1) :19-22.
[8]	崔广荣, 上官凌飞, 张子学, 等.文心兰类原球茎液体增殖过程中秋水仙素化学诱变[J].园艺学报, 2009, 16 (9) :1385-1389.
[9]	崔广荣, 张子学, 张从宇, 等.文心兰多倍体诱导及其鉴定[J].草业学报, 2010, 19 (1) :184-190.
[10]	叶秀仙, 黄敏玲, 吴建设等.文心兰茎尖诱导丛生芽高频率植株再生[J].福建农业学报, 2009, 24 (2) :126-137.
[11]	叶秀仙, 黄敏玲, 钟淮钦, 等.文心兰离体再生体系建立及试管苗种质保存研究.中国观赏园艺研究进展2009[M].北京:中国林业出版社, 2009:193-197.
[12]	肖军, 杨立国, 杨涛, 等.两种提取菊花总DNA的方法比较[J].辽宁农业科学, 2005, (1) :40-41.
[13]	刘进平, 郑成木.诱变结合植物组织培养在植物育种中的应用[J].上海农业学报, 2004, 20 (1) :19-22.
[14]	董颖苹, 连勇, 何庆才, 等.植物化学诱变技术在育种中的运用及其进展[J].种子, 2005, 24 (7) :54-58.
[15]	瞿素萍, 熊丽, 莫锡君, 等.香石竹的多倍体诱导及其变异研究[J].西南农业大学学报, 2004, (5) :609-612.
[16]	何林, 张洁, 郭启高, 等.东方百合Tiber多倍体诱导及其快繁研究[J].西南农业大学学报, 2006, (6) :945-949.
[17]	敬成俊, 李昌庭, 邓亚平, 等.秋水仙素间断式诱导植物多倍体研究[J].西南大学学报:自然科学版, 2013, 35 (7) :45-51.
[18]	张志胜, 谢利, 萧爱兴, 等.秋水仙素处理兰花原球茎对其生长和诱变效应的影响[J].核农学报, 2005, 19 (1) :19-23.
[19]	敬成俊, 李昌庭, 邓亚平, 等.秋水仙素间断式诱导植物多倍体研究[J].西南大学学报:自然科学版, 2013, 25 (7) :45-51.
[20]	FELIX L P, GUERRA M.Basic chromosome numbers of terrestrial orchids[J].Plant Systematics and Evolution, 2005, 254 (3) :131-148.