Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck

CHEN Hong-mei; CHEN Zhen; CHENG Long-fei; FU Guang-hua; SHI Shao-hua; WAN Chun-he; FU Qiu-ling; HUANG Yu

doi:10.19303/j.issn.1008-0384.2014.11.004

Volume 29 Issue 11

Nov. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(11): 1066-1069

CHEN Hong-mei, CHEN Zhen, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, WAN Chun-he, FU Qiu-ling, HUANG Yu. Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1066-1069. doi: 10.19303/j.issn.1008-0384.2014.11.004

Citation:

CHEN Hong-mei, CHEN Zhen, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, WAN Chun-he, FU Qiu-ling, HUANG Yu. Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1066-1069. doi: 10.19303/j.issn.1008-0384.2014.11.004

Citation:

CHEN Hong-mei, CHEN Zhen, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, WAN Chun-he, FU Qiu-ling, HUANG Yu. Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck[J]. Fujian Journal of Agricultural Sciences, 2014, 29(11): 1066-1069. doi: 10.19303/j.issn.1008-0384.2014.11.004

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Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck

doi: 10.19303/j.issn.1008-0384.2014.11.004

Animal Husbandry and Veterinary Institute,Fujian Academy of Agricultural Sciences/Fujian Animal Disease Control Technology Development Center

Received Date: 2014-09-03
Publish Date: 2014-11-18

Abstract

Abstract

A virus strain, named HeN1403, was isolated from the liver of dead Cherry Valley Breeding ducks.The virus killed 10 days Muscovy duck embryo within 79 hours and showed 33.3% of mortality rate to eight-day-old Muscovy ducklings.HeN1403 was characterized by specific RT-PCR primers which target to duck hepatitis virus A type 1, and it turned out to be a 200 bp fragment.A 714-bp fragment gene was obtained by using a set of primers aimed at VP1 ORF gene.Genetic analysis showed that the isolated virus was one of pancreatitis-type duck hepatitis virus A type 1.
- Cherry Valley Breeding duck,
- duck hepatitis virus a type 1,
- VP1gene

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References(13)

References

[1]	SAIF Y M.禽病学[M].苏敬良, 高福, 索勋, 译.12版.北京:中国农业出版社, 2012:431-436.
[2]	WANG L, PAN M, FU Y, et al.Classification of duck hepatitis virus into three genotypes based on molecular evolutionary analysis[J].Virus Genes, 2008, 37:52-59.
[3]	苏敬良, 黄瑜, 贺荣莲, 等.新型鸭肝炎病毒的分离及初步鉴定[J].中国兽医科技, 2002, 32 (1) :15-16.
[4]	杨维星, 施少华, 陈红梅, 等.3株鸭肝炎病毒1型全基因序列分析[J].中国农学通报, 2010, 26 (14) :8-12.
[5]	施少华, 程龙飞, 江斌, 等.韩国型鸭肝炎病毒福建株的分离与鉴定[J].畜牧与兽医, 2010, 42 (12) :9-12.
[6]	马秀丽, 宋敏训, 黄兵, 等.型鸭病毒性肝炎病毒RT-PCR检测方法的建立[J].家禽科学, 2006, (11) :11-13.
[7]	刘友生, 彭春香, 傅光华, 等.2010-2011年中国部分地区禽坦布苏病毒感染调查及分子变异分析[J].中国动物传染病学报, 2012, (1) :47-53.
[8]	PLUMMER P J, ALEFANTIS T, KAPLAN S, et al.Detection of duck enteritis virus by polymerase chain reaction[J].Avian Disease, 1998, 42 (3) :554-564.
[9]	傅光华, 施少华, 程龙飞, 等.种番鸭产蛋下降综合征病毒的分离及其PCR鉴定[J].福建农业学报, 2007, 22 (1) :43-45.
[10]	许秀梅, 苏敬良, 黄瑜, 等.两株鸭源禽呼肠孤病毒s3基因序列分析[J].中国兽医杂志, 2008, 44 (1) :12-14.
[11]	宋永峰, 温纳相, 宋延华, 等.应用PCR快速诊断番鸭细小病毒病和小鹅瘟[J].动物医学进展, 2009, 30 (5) :49-52.
[12]	TSENG C H, KNOWLES N J, TSAI H J.Molecular analysisof duck hepatitis virus type 1indicates that it should beassigned to a new genus[J].Virus Res, 2007, 123 (2) :190-203.
[13]	MUIR P, KAMMERER U, KOM K, et al.Molecular typing of enteroviruses:current status, future requirements[J].Clin Microbio Rev, 1998, 11 (1) :202-227.