Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.

ZHAO Yun-qing; CHEN Jing-ying; LIN Xiao-jun; LIU Bao-cai; HUANG Ying-zhen

doi:10.19303/j.issn.1008-0384.2014.10.012

Volume 29 Issue 10

Oct. 2014

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ZHAO Yun-qing, CHEN Jing-ying, LIN Xiao-jun, LIU Bao-cai, HUANG Ying-zhen. Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 995-999. doi: 10.19303/j.issn.1008-0384.2014.10.012

Citation:

ZHAO Yun-qing, CHEN Jing-ying, LIN Xiao-jun, LIU Bao-cai, HUANG Ying-zhen. Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 995-999. doi: 10.19303/j.issn.1008-0384.2014.10.012

Citation:

ZHAO Yun-qing, CHEN Jing-ying, LIN Xiao-jun, LIU Bao-cai, HUANG Ying-zhen. Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.[J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 995-999. doi: 10.19303/j.issn.1008-0384.2014.10.012

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Molecular Identification for Southern Blight Pathogen of Anoectoch ilusroxburghii(Wall.)Lindl.

doi: 10.19303/j.issn.1008-0384.2014.10.012

1. Institute of Agrobiological resources,Fujian Academy of Agricultural Sciences;
2. Medicinal Plant Research Center,Fujian academy of Agricultural Science;

Received Date: 2014-07-15
Publish Date: 2014-10-18

Abstract

Abstract

A suspected pathogen that caused the stalk rot on Anoectochilus roxburghii was isolated by means of tissue separation.It was subsequently subjected to the pathogenicity tests, morphological observation and molecular biology analysis for positive identification.The massive amount of aerial mycelia of the pathogen were gossypine, separate, branched, and capable of generating apurple pigment.Its macroconidium appeared in the shape of a spindle, and the microconidium in the shape of an egg.By inoculating the isolated strain to A.roxburghii, a pathogenicity to the plant was observed.Based on a blast test of rDNA-ITS sequence, the blight pathogen was identified to be Fusarium oxysporum Schl.
- Anoectochilus roxburghii (Wall.) Lindl.,
- stalk rot,
- pathogens,
- identification

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References(14)

References

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