Adaptation of Goose Parvovirus to Muscovy Duck Embryo Fibroblast

ZHU Chun-hua; HUANG Yu; FU Guang-hua; WAN Chun-he; CHENG Long-fei; FU Qiu-ling

doi:10.19303/j.issn.1008-0384.2014.09.001

Volume 29 Issue 9

Sep. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(9): 821-825

ZHU Chun-hua, HUANG Yu, FU Guang-hua, WAN Chun-he, CHENG Long-fei, FU Qiu-ling. Adaptation of Goose Parvovirus to Muscovy Duck Embryo Fibroblast[J]. Fujian Journal of Agricultural Sciences, 2014, 29(9): 821-825. doi: 10.19303/j.issn.1008-0384.2014.09.001

Citation:

ZHU Chun-hua, HUANG Yu, FU Guang-hua, WAN Chun-he, CHENG Long-fei, FU Qiu-ling. Adaptation of Goose Parvovirus to Muscovy Duck Embryo Fibroblast[J]. Fujian Journal of Agricultural Sciences, 2014, 29(9): 821-825. doi: 10.19303/j.issn.1008-0384.2014.09.001

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Adaptation of Goose Parvovirus to Muscovy Duck Embryo Fibroblast

doi: 10.19303/j.issn.1008-0384.2014.09.001

Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences

Received Date: 2014-04-02
Publish Date: 2014-09-18

Abstract

Abstract

In order to study the adaptation and propagation of goose parvovirus (GPV) in Muscovy duck embryo fibroblast (MDEF) , cultures through serial passages, and PCR assay, indirect immunofluorescence, flow cytometry were used to study the biological characteristics of GPV.Specified genomic nonstructural protein (NS) gene of GPV with the segment lengths of 734 bp was amplified by PCR, and PCR assays showed that the cell cultured 65 h after inoculate with 10-6 dilution of virus suspensions could be recognized specifically.Immunofluorescent studies showed that the infected MDEF had fluorescence characteristics using purified rabbit polyclonal antibody against GPV.The flow cytometry assay demonstrated that the GPV propagated slowly in MDEF, and GPV infection would induce the apoptosis in early stage.The results indicated the GPV strain adapted to and propagated in MDEF without cytopathic effects (CPE) on the cultures.The study provides a basis for cell dependent correlation research of GPV.
- GPV,
- MDEF,
- propagation,
- adaptation

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References(18)

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