Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory

YE Xue-jun; QIU Hong-duan; LIN Xin-jian; JIA Xian-bo; CHEN Ji-chen

doi:10.19303/j.issn.1008-0384.2014.08.011

Volume 29 Issue 8

Aug. 2014

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(8): 768-773

YE Xue-jun, QIU Hong-duan, LIN Xin-jian, JIA Xian-bo, CHEN Ji-chen. Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 768-773. doi: 10.19303/j.issn.1008-0384.2014.08.011

Citation:

YE Xue-jun, QIU Hong-duan, LIN Xin-jian, JIA Xian-bo, CHEN Ji-chen. Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 768-773. doi: 10.19303/j.issn.1008-0384.2014.08.011

Citation:

PDF( 1446 KB)

Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory

doi: 10.19303/j.issn.1008-0384.2014.08.011

1. College of Biological Science and Engineering,Fuzhou University;
2. Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences

Received Date: 2014-03-31
Publish Date: 2014-08-18

Abstract

Abstract

To investigate the diversity of mesophilic bacteria resulting from constant thermal conditions, the soil sample was collected at outfall of thermal wastewater from laundry factory.The culturable mesophilic bacteria were isolated with different medium to obtain pure culture, identified by 16 SrDNA and the data were analyzed in phylogenetic tree.The results showed that the 29 strains in pure culture were obtained from the thermal wastewater outfall soil with different phenotype.The strains were classified into Bacilli, attributed to 5genus and 11 species, including three species of Geobacillus, three species of Bacillus, three species of Anoxybacillus, one species of Ureibacillus, and one species of Thermoactinomyces.The diversity of species was rich, and the resources of species would have potential in practical application.
- laundry factory,
- culturable mesophilic bacteria,
- isolation,
- identification,
- diversity

FullText(HTML)

References(29)

References

[1]	LUND E, JOHN W.Characteristics, development and utilization of geothermal resources[J].Q Bull, 2007, 28:1-9.
[2]	刘艳, 黄晓航, 何培青, 等.印度洋深海热液区可培养细菌的分子鉴定与系统发育分析[J].海洋科学进展, 2009, 27 (2) :193-200.
[3]	陈志伟, 姜成英, 刘双江.云南和广东部分热泉Alicyclobacillus分布及系统发育[J].微生物学通报, 2004, 31 (3) :50-54.
[4]	刘东来, 蔡锦刚, 姚天羽, 等.长白山温泉无氧芽孢杆菌的分离鉴定[J].微生物学报, 2008, 48 (10) :1285-1289.
[5]	付烈, 周稳, 王俊卿, 等.嗜热石油降解菌YBW1的筛选、鉴定及性能研究[J].湖北工业大学学报, 2011, 26 (2) :84-87.
[6]	王大威, 张健, 齐义彬, 等.稠油降解菌的筛选及其对胶质降解作用[J].微生物学报, 2012, 52 (3) :353-359.
[7]	杨波, 陈新华.厦门地区近海温泉嗜热菌的分离、鉴定[J].台湾海峡, 2007, 26 (1) :61-70.
[8]	王春铭, 雷恒毅, 王国惠, 等.城市污泥模拟堆肥过程中高温菌群的筛选、鉴定及降解效果[J].环境科学学报, 2007, 27 (6) :979-986.
[9]	BERTUS VAN DEN BURG.Extremophiles as a source for novel enzymes[J].Current Opinion in Microbiology, 2003, 6 (3) :213-218.
[10]	RAMPELOTTO P H.Resistance of microorganisms to extreme environmental conditions and its contribution to astrobiology[J].Sustainability, 2010, 2 (6) :1602-1623.
[11]	PIKUTA E, LYSENKO A, CHUVILSKAYA N, et al.Anoxybacillus pushchinensis gen.nov., sp.nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb.nov[J].International Journal of Systematic and Evolutionary Microbiology, 2000, 50 (6) :2109-2117.
[12]	DEREKOVA A, SJHOLM C, MANDEVA R, et al.Anoxybacillus rupiensis sp.Nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria) [J].Extremophiles:Life Under Extreme Conditions, 2007, 11 (4) :577-583.
[13]	KEVBRIN V V, ZENGLER K, LYSENKO A M, et al.Anoxybacillus kamchatkensis sp.nov., a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley, Kamchatka[J].Extremophiles:Life Under Extreme Conditions, 2005, 9 (5) :391-398.
[14]	DAI J, LIU Y, LEI Y, et al.A new subspecies of Anoxybacillus flavithermus ssp.yunnanensis ssp.nov.with very high ethanol tolerance[J].FEMS Microbiology Letters, 2011, 320 (1) :72-78.
[15]	刘建玲, 雷毅, 阴耀邦, 等.1株嗜高温菌的分离、鉴定和免疫增强活性成分研究[J].西北农林科技大学学报:自然科学版, 2011, (8) :21-28, 33.
[16]	梁新乐, 杨龙, 张虹, 等.氡温泉中耐辐照嗜热菌的分离及其特性研究[J].核农学报, 2011, 25 (3) :488-493.
[17]	李建华, 张亚丽, 吕杰, 等.漠河盆地科学钻探井MK-1地下冷环境中嗜热微生物的多样性分析[J].冰川冻土, 2012, (3) :726-731.
[18]	林凤敏, 姬文秀, 尹祯焅, 等.长白山天池水可培养细菌的16SrRNA鉴定[J].延边大学农学学报, 2010, 32 (2) :77-81.
[19]	彭惠, 高毅, 肖亚中.一株高乙醇耐受的嗜热细菌Anoxybacillus sp.WP06的性质研究[J].生物工程学报, 2008, 24 (6) :1117-1120.
[20]	GATSON J W, BENZ B F, CHANDRASEKARAN C, et al.Bacillus tequilensis sp.nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis[J].International Journal of Systematic and Evolutionary Microbiology, 2006, 56 (7) :1475-1484.
[21]	HEYRMAN J, VANPARYS B, LOGAN N A, et al.Bacillus novalis sp.nov., Bacillus vireti sp.nov., Bacillus soli sp.nov., Bacillus bataviensis sp.nov.and Bacillus drentensis sp.nov., from the Drentse A grasslands[J].International Journal of Systematic and Evolutiona, 2004, 54 (1) :47-57.
[22]	NAZINA T N, TOUROVA T P, POLTARAUS A B, et al.Taxonomic study of aerobic thermophilic bacilli:descriptions of Geobacillus subterraneus gen.nov., sp.nov.and Geobacillus uzenensis sp.nov.from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G.stearothermophilus, G.th[J].International Journal of Systematic and Evolutionary Microbiology, 2001, 51 (2) :433-446.
[23]	董永存, 刘洋, 陈源源, 等.嗜热菌来源的生淀粉酶分离纯化及其酶学性质[J].微生物学报, 2008, (2) :169-175.
[24]	晋果果, 翁海波, 李萍萍, 等.高温木质素降解菌Geobacillus caldoxylosilyticus J16的筛选及其产酶发酵性质研究[J].中国农学通报, 2011, (8) :334-339.
[25]	夏文杰, 董汉平, 俞理.嗜热耐盐烃降解菌Geobacillus sp.WJ-2降解原油性能研究[J].中南大学学报:自然科学版, 2012, (1) :8-16.
[26]	FORTINA M G, PUKALL R, SCHUMANN P, et al.Ureibacillus gen.nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al.1995) , emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp.nov[J].International Journal of Systematic and Evolutionary Microbiology, 2001, 51 (2) :447-455.
[27]	王伟东, 王小芬, 李玉花, 等.复合系WSC-6的菌种组成特性及其木质纤维素分解能力[J].农业工程学报, 2007, (10) :210-215.
[28]	解开治, 徐培智, 张发宝, 等.接种微生物菌剂对猪粪堆肥过程中细菌群落多样性的影响[J].应用生态学报, 2009, (8) :2012-2018.
[29]	陶天申, 杨瑞馥, 东秀珠.原核生物系统学[M].北京:化学工业出版社, 2007:572-573.