Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae

FAN Rong-hui; HUANG Min-ling; ZHONG Huai-qin; WU Jian-she

doi:10.19303/j.issn.1008-0384.2014.08.008

Volume 29 Issue 8

Aug. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(8): 748-751

FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she. Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008

Citation:

FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she. Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae[J]. Fujian Journal of Agricultural Sciences, 2014, 29(8): 748-751. doi: 10.19303/j.issn.1008-0384.2014.08.008

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Cloning and Expression Analysis of Dihydroflavonol 4-reductase in Strelitzia reginae

doi: 10.19303/j.issn.1008-0384.2014.08.008

1. Institute of Crop Sciences,Fujian Academy of Agricultural Science;
2. Flowers Research Center,Fujian Academy of Agricultural Science;
3. Fujian Engineering Research Center for Characteristic Floriculture

Received Date: 2014-05-12
Publish Date: 2014-08-18

Abstract

Abstract

The SrDFRgene cDNA sequence involved in flavonoids synthesis was cloned from the blue petals of strelitzia reginae using RT-PCR techniques.The cDNA sequence consists of 246 bp, encoding apolypeptide of 82 amino acids.Homology analysis showed that the deduced SrDFRprotein was highly homologous to other DFR proteins from different plants.Phylogenetic analysis indicated that SrDFR was clustered together with DFR of Curcuma alismatifoliafirstly and had close genetic relationship.The semi-quantitative PCR analysis indicated that SrDFRshowed the highest transcript abundance in e and SrDFR was highly expressed in blue petals, but lowly expressed in leaves and yellow sepals.The results indicated that SrDFRplayed an important role in the growth of blue petals.
- strelitzia reginae,
- dihydroflavonol 4-reductase,
- flavonoids synthesis,
- gene cloning

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References(14)

References

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