Isolation and Characterisation of a Testosterone-Degrading Bacterium

BAI Ze-fang; PAN Da-ren; HUO Yu-jing; LI Qing

doi:10.19303/j.issn.1008-0384.2014.07.019

Volume 29 Issue 7

Jul. 2014

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BAI Ze-fang, PAN Da-ren, HUO Yu-jing, LI Qing. Isolation and Characterisation of a Testosterone-Degrading Bacterium[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 698-701. doi: 10.19303/j.issn.1008-0384.2014.07.019

Citation:

BAI Ze-fang, PAN Da-ren, HUO Yu-jing, LI Qing. Isolation and Characterisation of a Testosterone-Degrading Bacterium[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 698-701. doi: 10.19303/j.issn.1008-0384.2014.07.019

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Isolation and Characterisation of a Testosterone-Degrading Bacterium

doi: 10.19303/j.issn.1008-0384.2014.07.019

1. College of Crop Science,Fujian Agriculture and Forestry University;
2. College of Life Sciences,Fujian Agriculture and Forestry University

Received Date: 2014-05-03
Publish Date: 2014-07-18

Abstract

Abstract

One bacterium, which has testosterone degradation efficiently, was isolated from the sludge, Fujian and named C53-20.The results of morphological characteristics and the similarity analysis of the 16 SrDNA sequence suggest that it is a Klebsiella pneumonia.In addition, testosterone degradation activity in 35-40℃and pH6.0-8.0range was assayed, and our results suggest that the optimum activity is at 37℃and pH6.5, respectively.Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45%, with1mmol·L-1 concentrations within 12 hours.Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.
- degrading bacteria,
- testosterone,
- 16S rDNA

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References(17)

References

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