Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15

ZHAO Xiao-yan; WU Xiao-qing; ZHAO Zhong-juan; CHEN Quan; LI Zhe; LI Ji-shun; YANG He-tong

doi:10.19303/j.issn.1008-0384.2014.07.013

Volume 29 Issue 7

Jul. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(7): 669-672

ZHAO Xiao-yan, WU Xiao-qing, ZHAO Zhong-juan, CHEN Quan, LI Zhe, LI Ji-shun, YANG He-tong. Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 669-672. doi: 10.19303/j.issn.1008-0384.2014.07.013

Citation:

ZHAO Xiao-yan, WU Xiao-qing, ZHAO Zhong-juan, CHEN Quan, LI Zhe, LI Ji-shun, YANG He-tong. Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 669-672. doi: 10.19303/j.issn.1008-0384.2014.07.013

Citation:

ZHAO Xiao-yan, WU Xiao-qing, ZHAO Zhong-juan, CHEN Quan, LI Zhe, LI Ji-shun, YANG He-tong. Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 669-672. doi: 10.19303/j.issn.1008-0384.2014.07.013

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Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB15

doi: 10.19303/j.issn.1008-0384.2014.07.013

Shandong Provincial Key Laboratory of Applied Microbiology,China-Japan Friendship Biotechnology Research Center

Received Date: 2014-04-05
Publish Date: 2014-07-18

Abstract

Abstract

The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37) .The results showed that the optimum fermentation culture medium contained 3.0% corm meal, 2.0% soybean powder, 1.0% sugar, 0.1% ammonium sulfate, 0.3% potassium hydrogen phosphate trihydrate, 0.2% potassium dihydrogen phosphate, and 0.04% magnesium sulfate heptahydrate.The number of viable cells was 4.52×109 cfu·mL-1and the rate of spore formation was 90%after 48hs′fermentation.The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua were 66.93%and 69.07%, respectively.
- fermentation,
- medium optimization of,
- orthogonal experiment,
- strain TLB15,

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References(9)

References

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