RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice

HE Wei; ZHU Yong-sheng; LIAN Ling; ZHOU Ping; CAI Qiu-hua; WANG Ying-heng; XIE Hong-guang; ZHANG Jian-fu; XIE Hua-an

doi:10.19303/j.issn.1008-0384.2014.07.007

Volume 29 Issue 7

Jul. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(7): 641-645

HE Wei, ZHU Yong-sheng, LIAN Ling, ZHOU Ping, CAI Qiu-hua, WANG Ying-heng, XIE Hong-guang, ZHANG Jian-fu, XIE Hua-an. RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 641-645. doi: 10.19303/j.issn.1008-0384.2014.07.007

Citation:

HE Wei, ZHU Yong-sheng, LIAN Ling, ZHOU Ping, CAI Qiu-hua, WANG Ying-heng, XIE Hong-guang, ZHANG Jian-fu, XIE Hua-an. RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 641-645. doi: 10.19303/j.issn.1008-0384.2014.07.007

Citation:

HE Wei, ZHU Yong-sheng, LIAN Ling, ZHOU Ping, CAI Qiu-hua, WANG Ying-heng, XIE Hong-guang, ZHANG Jian-fu, XIE Hua-an. RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 641-645. doi: 10.19303/j.issn.1008-0384.2014.07.007

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RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice

doi: 10.19303/j.issn.1008-0384.2014.07.007

HE Wei^1,2,3,4,5,
ZHU Yong-sheng^1,2,3,4,5,
LIAN Ling^1,2,3,4,5,
ZHOU Ping⁶,
CAI Qiu-hua^1,2,3,4,5,
WANG Ying-heng^1,2,3,4,5,
XIE Hong-guang^1,2,3,4,5,
ZHANG Jian-fu^1,2,3,4,5,
XIE Hua-an^1,2,3,4,5

1. Rice Research Institute,Fujian Academy of Agricultural Sciences;
2. Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture,P.R China/Fuzhou branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding;
3. Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology;
4. National Rice Engineering Laboratory of China;
5. South China Bases of National Key Laboratory of Hybrid Rice for China;
6. Fruit Research Institute,Fujian Academy of Agricultural Sciences

Received Date: 2014-03-10
Publish Date: 2014-07-18

Abstract

Abstract

Based on the previously obtained overexpression transgenic plants with the ubiquitin ligase, OsHUB2, from rice, expression lever of the enzyme was studied.Three pairs of primers, OsHUB2-F1R1, OsHUB2-F2R2 and OsHUB2-F3R3, were designed using the Primer Premier 5.0according to the sequence of the enzyme from GeneBank.Subsequently, RT-PCR was conducted to select the best primer pairs with a specific and single amplification product.The results showed OsHUB2-F3R3 to be desirable on both and functions.The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase.
- rice,
- ubiquitin ligase OsHUB2,
- RT-PCR (qRT-PCR)

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References(13)

References

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