The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus

WANG Long-bo; CHE Yong-liang; WU Xue-min; CHEN Ru-jing; LIU Yu-tao; ZHOU Lun-jiang

doi:10.19303/j.issn.1008-0384.2014.07.002

Volume 29 Issue 7

Jul. 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(7): 618-622

WANG Long-bo, CHE Yong-liang, WU Xue-min, CHEN Ru-jing, LIU Yu-tao, ZHOU Lun-jiang. The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 618-622. doi: 10.19303/j.issn.1008-0384.2014.07.002

Citation:

WANG Long-bo, CHE Yong-liang, WU Xue-min, CHEN Ru-jing, LIU Yu-tao, ZHOU Lun-jiang. The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 618-622. doi: 10.19303/j.issn.1008-0384.2014.07.002

Citation:

WANG Long-bo, CHE Yong-liang, WU Xue-min, CHEN Ru-jing, LIU Yu-tao, ZHOU Lun-jiang. The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(7): 618-622. doi: 10.19303/j.issn.1008-0384.2014.07.002

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The Establishment and Preliminary Application of a RT-PCR for Detection of Swine Lnfluenza Virus

doi: 10.19303/j.issn.1008-0384.2014.07.002

Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences/Animal Disease Control Technology Development Center

Received Date: 2014-04-21
Publish Date: 2014-07-18

Abstract

Abstract

A pair of primers was designed based on the M gene sequence of the Swine influenza virus (SIV) published in Genbank, and the matched RT-PCR assay was developed.The 675 bp specific fragment was amplified from H1N1, H1N2 and H3N2subtype Swine influenza viruses.Negative results were found on PRV, CSFV, PCV2 and PRRSV.The developed method was sensitive to detect RNA as little as 3.5pg, and Siv could be detected directly from lungs of infected mice and pathogenic swine.This RT-PCR assay was proved to be specific, sensitive, reproducible and suitable for rapid diagnosis of H1N1, H1N2 and H3N2.
- swine Influenza virus,
- RT-PCR,
- establishment

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References(11)

References

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