Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid

GUO Lin-wei; CHEN Min; ZHANG Xiang

doi:10.19303/j.issn.1008-0384.2014.06.016

Volume 29 Issue 6

Jun. 2014

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GUO Lin-wei, CHEN Min, ZHANG Xiang. Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 592-596. doi: 10.19303/j.issn.1008-0384.2014.06.016

Citation:

GUO Lin-wei, CHEN Min, ZHANG Xiang. Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 592-596. doi: 10.19303/j.issn.1008-0384.2014.06.016

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Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid

doi: 10.19303/j.issn.1008-0384.2014.06.016

1. Fujian Agriculture and Forestry University;
2. Fujian Academy of Agricultural Sciences

Received Date: 2014-02-20
Publish Date: 2014-06-18

Abstract

Abstract

For studying the simulated microgravity in rotating bioreactor and the movement of micro-carrier in the microgravity fluid, two methods were applied to verify the effect of simulated microgravity in rotating bioreactor:keeping the effective acceleration of gravity far less than the surface gravity acceleration, or the " approximate static" condition for micro-carrier in the aqueous solution.In the self-developed perfusion system in rotating bioreactor, the motion displacement and speed of micro-carrier were analyzed to find out the movement rules of micro-carrier in the aqueous solution, and the ideal radius of membrane oxygenator in cell bottle was decided by centrifugal force calculating.The results showed that micro-carrier moved from center to outside following a certain rule until touching the wall.To get better test results, the radius of membrane oxygenator was set to be a small parameter between 0.3 733-0.8 042 cm.
- simulated microgravity,
- perfusion system,
- micro-carrier movement

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References(1)

References

[1]

]齐乃明, 张文辉, 高九州, 等.空间微重力环境地面模拟试验方法综述[J].航天控制, 2011, 29 (3) :95-100.[5]JIANG PIDONG.Space Biology[M].Qingdao:Qingdao Publishers, 2000:476-484.[6]VAN LOON J J W A.The use of instruments for gravity related research[R].COSPAR 2006-A-03806, 2006:7.[7]ZENG YE, LIU XIAO-HENG.Experimental technologies for imposing mechanical force on cell[J].Space Medicine&MedicaI Engineering, 2007, 20 (3) :231-232.[8]陈敏, 邓素芳, 杨有泉.二轴植物旋转栽培装置[P].中国专利:ZL200710009491.6, 2010-6-30.[9]陈敏, 刘润东, 杨有泉, 等.红萍湿养栽培供O2装置研制[J].空间科学学报, 2010, 30 (2) :185-192.[10]陈敏, 邓素芳, 杨有泉, 等.受控生态生报系统内红萍供养特性研究[J].空间科学学报, 2012, 32 (2) :223-228.[11]陈敏, 杨有泉, 邓素芳, 等.旋转式植物湿润栽培供水装置[P].中国专利:ZL200710009492.0, 2010-09-29.[12]胡文瑞.微重力科学概论[M].北京:科学出版社, 2011:1-2.[13]ALBRECHT-BUEHLER G.Possible mechanisms of indirect gravity sensing by cell[J].ASGSB Bull, 1991, 4 (2) :25-34.[14]童幸生, 张静.氧化铝陶瓷摩擦磨损实验研究[J].江汉大学学报:自然科学版, 2011, (2) :34-37.[15]WOLF D A, SCHWARZ R P.Analysis of gravity-induced particle motion and fluid perfusion flow in the NASA-designed rotating zero head space tissue culture vessel[J].NASA Technical Paper, 1991, 3143:1-12.[16]马萧, 杨春.壁式慢回转培养器中的微载体运动特点[J].生物医学工程杂志, 2010, 27 (6) :1434-1438.[17]POLAC K S R, MEANEY D F, LEVINE E M, et al.Numerical model and experimental validation of microcarrier motion in a rotating bioreactor[J].Tissue Engineering, 2000, 6 (5) :519-530.[18]JOHNSSON A, PICKARD B G.Threshold stimulus for geotropism[J].Physiol Plantarum, 1979, 45 (3) :315-319.[19]VAN LOON J J W A.Some history and use of the random positioning machine, rpm, in gravity related research[J].Adv Space Res, 2007, (7) :1161-1165.

SONNENFELD G.The immune system in space and microgravity[J].Med Sci Sports Exerc, 2002, 34 (12) :2021-2027.[2]ALMEIDA E.The effects of microgravity on cells[J].NASA Report, 2002.[3]TANI A, OKUMA T, GOTO E, et al.Ground performance of air conditioning and water recycle system for a space plant box[J].Adv Space Res, 2001, 27 (9) :1557-1562.[4

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