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Volume 29 Issue 6
Jun.  2014
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Article Contents
CHE Jian-mei, LIU Bo, RUAN Chuan-qing, WANG Guo-fen, HUANG Dan-dan, YANG Zhong-lai, GUO Hui-hui. Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 580-585. doi: 10.19303/j.issn.1008-0384.2014.06.014
Citation: CHE Jian-mei, LIU Bo, RUAN Chuan-qing, WANG Guo-fen, HUANG Dan-dan, YANG Zhong-lai, GUO Hui-hui. Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 580-585. doi: 10.19303/j.issn.1008-0384.2014.06.014

Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics

doi: 10.19303/j.issn.1008-0384.2014.06.014
  • Received Date: 2013-09-28
  • Publish Date: 2014-06-18
  • In order to identify the causal agent of black spot disease on wax apple (Syzygium samarangense Merr.et Perry) in its taxonomic status, the causal fungi FJAT-9860 was isolated and characterized with pathogenicity test, morphology and culture feature observation, rDNA-ITS sequences assay and biological characteristics test.The results indicated that the causal agent belong to Lasiodiplodia theobromae.The colony of strain FJAT-9860 on PDA was white at first, then gradually became as grey to black.The monadic conidia were elliptic to round, and colourless.The rDNA-ITS sequence of the strain shared 99%identity with that of Lasiodiplodia theobromae strain.The results showed that the optimum temperature for mycelium growth of this pathogen was 25℃and the optimum pH was 8.0.The mycelial growth was affected by different carbon sources and the best one was mannitol.
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