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Volume 29 Issue 6
Jun.  2014
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Article Contents
CHE Jian-mei, LIU Bo, RUAN Chuan-qing, WANG Guo-fen, HUANG Dan-dan, YANG Zhong-lai, GUO Hui-hui. Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 580-585. doi: 10.19303/j.issn.1008-0384.2014.06.014
Citation: CHE Jian-mei, LIU Bo, RUAN Chuan-qing, WANG Guo-fen, HUANG Dan-dan, YANG Zhong-lai, GUO Hui-hui. Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 580-585. doi: 10.19303/j.issn.1008-0384.2014.06.014

Identification Pathogen Causing Black Spot Disease on Wax Apple(Syzygium samarangense Merr.et Perry)and its Biological Characteristics

doi: 10.19303/j.issn.1008-0384.2014.06.014
  • Received Date: 2013-09-28
  • Publish Date: 2014-06-18
  • In order to identify the causal agent of black spot disease on wax apple (Syzygium samarangense Merr.et Perry) in its taxonomic status, the causal fungi FJAT-9860 was isolated and characterized with pathogenicity test, morphology and culture feature observation, rDNA-ITS sequences assay and biological characteristics test.The results indicated that the causal agent belong to Lasiodiplodia theobromae.The colony of strain FJAT-9860 on PDA was white at first, then gradually became as grey to black.The monadic conidia were elliptic to round, and colourless.The rDNA-ITS sequence of the strain shared 99%identity with that of Lasiodiplodia theobromae strain.The results showed that the optimum temperature for mycelium growth of this pathogen was 25℃and the optimum pH was 8.0.The mycelial growth was affected by different carbon sources and the best one was mannitol.
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  • [1]
    陈惠宗.莲雾高产优质栽培技术[J].福建农业, 2012, (2) :19.[2]叶建兵, 陈发河, 吴光斌.一氧化氮对莲雾果实采后生理及品质的影响[J].集美大学学报:自然科学版, 2012, 17 (3) :180-185.[3]杨凤珍, 李敏, 高兆银, 等.莲雾果实病害及防治研究进展[J].浙江农业科学, 2009, (5) :961-964.[4]郑俊雄, 陈惠宗.台湾黑珍珠莲雾优质高产栽培技术[J].台湾农业探索, 2011, (5) :21-22.[5]黄蓬英, 黄何何, 洪钦阳, 等.台湾“黑珍珠”莲雾反季节栽培技术实践[J].江西农业学报, 2011, 23 (1) :56-57.[6]方中达.植病研究方法[M].第3版.北京:中国农业出版社, 1998.[7]刘少华, 陆金萍, 朱瑞良, 等.一种快速简便的植物病原真菌基因组DNA提取方法[J].植物病理学报, 2005, 35 (4) :362-365.[8]ALVES A, CROUS P W, CORREIA A, et al.Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae[J].Fungal Diversity, 2008, 28:1-13.[9]MUHAMMAD S, ZAFAR I, AHMAD S, et al.Association of Lasiodiplodia theobromae with different decline disorders in Mango (Mangifera indica L.) [J].Pak J Bot, 2009, 41 (1) :359-368.[10]胡美姣, 高兆银, 李敏, 等.杧果果实潜伏侵染真菌种类研究[J].果树学报, 2012, 29 (1) :105-110.[11]张传飞, 姜子德, 钟国强, 等.外源水果储运期病害研究初报[J].仲恺农业技术学院学报, 2005, 18 (4) :53-57.[12]张居念, 林河通, 谢联辉, 等.龙眼果实潜伏性病原真菌的初步研究[J].热带作物学报, 2006, 27 (4) :78-82.[13]BALI R V, BINDU M G, CHENGA R V, et al.Postharvest fungal spoilage in sweet orange (Citrus sinensis) and acid lime (Citrus aurentifolia Swingla) at different stages of marketing[J].Agric Sci Digest, 2008, 28:265-267.[14]MOHALI S, BURGESS T I, WINGFIELD M J.Diversity and host association of the tropical tree endophyte Lasiodiplodia theobromae revealed using simple sequence repeat markers[J].Forest Pathology, 2005, 35:385-396.[15]TREENA I B, PAUL A B, SARI M, et al.Three new Lasiodiplodia spp.from the tropics, recognized based on DNA sequence comparisons and morphology[J].Mycologia, 2006, 98 (3) :423-435.[16]张志华, 洪葵.29株具有抗菌和细胞毒活性红树林真菌的鉴定[J].热带作物学报, 2010, 31 (9) :1647-1654.

    [17]MILLER A N, HUHNDORF S M.Using phylogenetic species recognition to delimit species boundaries within Lasiosphaeria[J].Mycologia, 2004, 96:1106-1127.
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