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Volume 29 Issue 6
Jun.  2014
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Article Contents
LIN Jiang-bo, WANG Wei-ying, ZOU Hui, LI Hai-ming, WU Shui-jin, DAI Yi-min. Cloning and Sequence Analysis of Chloroplast ATP Synthase Gene NtATPFfrom Narcissus tazetta var.Chinensis[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 546-549. doi: 10.19303/j.issn.1008-0384.2014.06.007
Citation: LIN Jiang-bo, WANG Wei-ying, ZOU Hui, LI Hai-ming, WU Shui-jin, DAI Yi-min. Cloning and Sequence Analysis of Chloroplast ATP Synthase Gene NtATPFfrom Narcissus tazetta var.Chinensis[J]. Fujian Journal of Agricultural Sciences, 2014, 29(6): 546-549. doi: 10.19303/j.issn.1008-0384.2014.06.007

Cloning and Sequence Analysis of Chloroplast ATP Synthase Gene NtATPFfrom Narcissus tazetta var.Chinensis

doi: 10.19303/j.issn.1008-0384.2014.06.007
  • Received Date: 2014-01-12
  • Publish Date: 2014-06-18
  • Based on a fragment cDNA from suppression subtractive hybridization (SSH) of Narcissus tazetta var.chinensis induced by paclobutrazol, an ATP Synthase CF0 subunit gene NtATPF was separated and cloned by RACE and RT-PCR.The full length of NtATPFcDNA was 905 bp with a 555 bp ORF, which encoded a protein of184 amino acids and had a transmembrance domain (27-49) with a predicted molecular mass of 20.809 5kD and theoretical isoelectric point of 5.69.Phylogenetic analysis showed that NtATPF had close relationship to ATP Synthase CF0 subunit in Allium fistulosum (AEX93661.1) .
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