A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe

LIU Bao-cai; HUANG Ying-zhen; ZHAO Yun-qing; CHEN Jing-ying

doi:10.19303/j.issn.1008-0384.2014.05.011

Volume 29 Issue 5

May 2014

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(5): 461-464

LIU Bao-cai, HUANG Ying-zhen, ZHAO Yun-qing, CHEN Jing-ying. A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 461-464. doi: 10.19303/j.issn.1008-0384.2014.05.011

Citation:

LIU Bao-cai, HUANG Ying-zhen, ZHAO Yun-qing, CHEN Jing-ying. A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 461-464. doi: 10.19303/j.issn.1008-0384.2014.05.011

Citation:

PDF( 1027 KB)

A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe

doi: 10.19303/j.issn.1008-0384.2014.05.011

1. Research Center for Medicinal Plant,Fujian Academy of Agricultural Sciences;
2. Institute of Agricultural Bioresource,Fujian Academy of Agricultural Sciences

Received Date: 2014-01-14
Publish Date: 2014-05-18

Abstract

Abstract

To alleviate a seed shortage on Pholidota cantonensis Rolfe, artificial culturing with medium optimization was investigated by using the plant tissue culture technology.The results showed that MS+20g·L-1 sucrose+8g·L-1 agar was an appropriate medium that allowed a germination rate above 70%.MS+50g·L-1 potato+20g·L-1 sucrose+8g·L-1 agar was desirable for the plantlet′s protocorm differentiation.Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation.The medium formulated with 1/2MS+0.5mg·L-1 NAA+20g·L-1 sucrose+8g·L-1 agar was optimal for rooting, as a rate of 100%was achieved.By using the combination of the above-mentioned media at respective stages, the plantlets of P.cantonensis Rolfe could be rapidly obtained from seeds.
- Pholidota cantonensis Rolfe,
- seed,
- aseptic seeding,
- rapid propagation

FullText(HTML)

References(13)

References

[1]	陈心启, 吉占和, 郎楷永, 等.中国植物志[M].18卷.北京:科学出版社, 1999:392-393.
[2]	林来官, 张永田.福建植物志[M].第六卷.福州:福建科学技术出版社, 1994:636-638.
[3]	王莲辉, 姜运力.尖叶石仙桃的胚培养与快速繁殖[J].植物生理学通讯, 2006, 42 (5) :136-137.
[4]	曾碧玉, 朱根发, 刘海涛, 等.4种野生兰花种子特征及离体培养初报[J].亚热带植物科学, 2008, 37 (3) :31-34.
[5]	廖彭莹, 李兵, 苗伟生, 等.细叶石仙桃挥发油成分的GC-MS分析[J].安徽农业科学, 2011, 39 (22) :13394-13395.
[6]	沈宗根, 吕洪飞, 程存归.3种石斛属植物和细叶石仙桃的红外光谱分析和鉴定[J].西北植物学报, 2008, 28 (1) :97-102.
[7]	祁献芳, 李东, 康文艺.细叶石仙桃地上部分脂溶性成分GCMC分析[J].河南大学学报:医学版, 2011, 30 (2) :90-92.
[8]	建晨, 冯丽, 野原稔弘.细叶石仙桃的化学成分[J].中国中药杂志, 2008, 33 (14) :1691-1693.
[9]	丽聪, 吴春敏, 陈海滨, 等.RP-HPLC法测定石仙桃中天麻素和天麻苷元[J].中草药, 2008, 39 (2) :283-285.
[10]	林丽聪, 张怡平, 吴春敏, 等.石仙桃叶化学成分研究[J].时珍国医国药, 2009, 20 (4) :922-923.
[11]	LIU J X, ZHOU L, ZHOU Q, et al.Animal Experiment of the Fatigue-resisting and Anoxia-resisting Actions of Pholidota Chinensis Lindl[J].Chinese Journal of Clinical Rehabilitation, 2006, 10 (7) :157-159.
[12]	刘洪旭, 吴春敏, 林丽聪, 等.石仙桃镇痛有效提取部位研究[J].福建中医学院学报, 2004, 14 (4) :34-36.
[13]	刘建新, 周青, 连其深.石仙桃的镇痛作用的研究[J].赣南医学院学报, 2002, 22 (2) :105-107.