Studying on Somatic Embryo Induction of Fortunella japonica

HUANG Jing; CHEN Xiao-jing

doi:10.19303/j.issn.1008-0384.2014.05.010

Volume 29 Issue 5

May 2014

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HUANG Jing, CHEN Xiao-jing. Studying on Somatic Embryo Induction of Fortunella japonica[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 454-460. doi: 10.19303/j.issn.1008-0384.2014.05.010

Citation:

HUANG Jing, CHEN Xiao-jing. Studying on Somatic Embryo Induction of Fortunella japonica[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 454-460. doi: 10.19303/j.issn.1008-0384.2014.05.010

HUANG Jing, CHEN Xiao-jing. Studying on Somatic Embryo Induction of Fortunella japonica[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 454-460. doi: 10.19303/j.issn.1008-0384.2014.05.010

Citation:

HUANG Jing, CHEN Xiao-jing. Studying on Somatic Embryo Induction of Fortunella japonica[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 454-460. doi: 10.19303/j.issn.1008-0384.2014.05.010

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Studying on Somatic Embryo Induction of Fortunella japonica

doi: 10.19303/j.issn.1008-0384.2014.05.010

HUANG Jing¹,
CHEN Xiao-jing²

1. Fujian Agricultural Vocational and Technical College Department of Horticulture and Lanscape;
2. College of Horticulture,Fujian Agriculture and Forestry University

Received Date: 2013-12-30
Publish Date: 2014-05-18

Abstract

Abstract

Based on single factorial experiment, the sterile seedlings of Fortunella japonica were used as explants for studying the effects of various growth regulators on inducing embryogeny with orthogonal test.The results showed:the MS+2, 4-D 2.0mg·L-1+BA 0.5mg·L-1 was the best culture medium for inducing embryonic callus.Sucrose at MS culture medium with BA 0.05mg·L-1 gave a maximum embryogenesis from the light yellow callus tissue of leaf.Planting somatic embryos on 1/2MS culture medium with NAA0.1mg·L-1, the rooting rate was 80%, the survive rate was 70%.
- Fortunella japonica,
- tissue culture,
- somatic embryo

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References(5)

References

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