• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 29 Issue 5
May  2014
Turn off MathJax
Article Contents
XU Li-juan, ZHOU Hong-zhang, ZHANG Shu-ning, WANG Ya-mei, ZHENG Yu-li. Induction and Identification for Autotetraploid Non-heading Chinese Cabbage in High-quality and Heat-tolerance[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 449-453. doi: 10.19303/j.issn.1008-0384.2014.05.009
Citation: XU Li-juan, ZHOU Hong-zhang, ZHANG Shu-ning, WANG Ya-mei, ZHENG Yu-li. Induction and Identification for Autotetraploid Non-heading Chinese Cabbage in High-quality and Heat-tolerance[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 449-453. doi: 10.19303/j.issn.1008-0384.2014.05.009

Induction and Identification for Autotetraploid Non-heading Chinese Cabbage in High-quality and Heat-tolerance

doi: 10.19303/j.issn.1008-0384.2014.05.009
  • Received Date: 2014-01-16
  • Publish Date: 2014-05-18
  • Using diploid non-heading Chinese cabbage‘Yi-ling', the apical portion of their seedlings were treated with colchicine solution in different concentration.The variant plants were selected and identified in their ploidy, and were also studied in their characters of morphology, anatomy, cytology, their heat-tolerance, agronomic and nutritional traits.The results indicated that the treatment in colchicine 0.2 mol·L-1 for 4times resulted of tetraploid doubling in 7.64%.Comparing with the diploid plant, the tetraploid plants were significantly increased in the length and width of leaf and petioles, the number of flowering organs, stomata and pollen, the weight of leaf, petioles and plant, while setting percentage was receded remarkably.Based on the diploid plants, the contents of total soluble sugar, soluble protein and dry matter in autotetraploid plants were increased by 7.32%, 74.85%and3.91% for the leaves, and by 17.22%, 1.94% and 4.89% for the petioles, respectively.Similarly in the comparison, the content of organic acids in autotetraploid plant leaves and petioles was decreased significantly by22.56% and 25.00%, respectively.Under 42℃ heating stress, the content of MDA and free proline were significantly lower while soluble protein was higher in autotetraploid plants than in diploid plants.The results indicated that autotetraploid plants performed in high-quality and heat-tolerance.
  • loading
  • [1]
    刘惠吉, 曹寿椿, 王华, 等.南农矮脚黄四倍体不结球白菜新品种的选育[J].南京农业大学学报, 1990, 13 (2) :33-40.
    [2]
    刘惠吉, 王华, 肖守华, 等.四倍体热优2号的选育[J].南京农业大学学报, 1992, 15 (4) :39-44.
    [3]
    徐丽娟, 张蜀宁, 郑于莉, 等.秋水仙素诱导不结球白菜叶片形态类型与染色体加倍的关系[J].西北植物学报, 2013, 33 (6) :1239-1244.
    [4]
    水德聚, 石瑜, 曹亮亮, 等.外源水杨酸预处理对高温胁迫下白菜耐热性和光合特性的影响[J].植物生理学报, 2012, 48 (4) :386-392.
    [5]
    张振超, 张蜀宁, 张伟, 等.四倍体不结球白菜的诱导及染色体倍性鉴定[J].西北植物学报, 2007, 27 (1) :28-32.
    [6]
    郑金双, 张蜀宁, 不结球白菜根尖细胞染色体制片及其二倍体和四倍体有丝分裂过程观察[J].西北资源与环境学报, 2011, 20 (4) :58-63.
    [7]
    郑金双, 张蜀宁.二倍体及同源四倍体不结球白菜核型分析[J].南京农业大学学报, 2012, 35 (1) :131-134.
    [8]
    王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2006:186-280.
    [9]
    崔秀敏, 张义凯, 王秀峰, 等.不结球白菜品质性状与矿质营养的典型相关及逐步回归分析[J].华北农学报, 2008, 23 (S1) :25-28.
    [10]
    曾国平, 曹寿椿.不结球白菜主要品质性状遗传效应分析[J].园艺学报, 1997, 24 (1) :43-47.
    [11]
    常金华, 罗耀武, 王宝义.同源四倍体高粱育性的改良及利用研究[J].华北农学报, 1999, 14 (S1) :24-27.
    [12]
    胡俏强, 袁希汉.不结球白菜抗热性鉴定方法及其抗热基因片段克隆研究[D].南京:南京农业大学园艺学院, 2011.
    [13]
    叶陈亮, 柯玉琴, 陈伟.大白菜抗热性的生理研究*Ⅱ.叶片水分和蛋白质代谢与抗热性[J].福建农业大学学报, 1996, 25 (4) :490-493.
    [14]
    王敏, 曲存民, 刘晓兰, 等.温度胁迫下甘蓝型油菜苗期生理生化指标的研究[J].作物杂志Crops, 2013, (2) :53-59.
    [15]
    LIU S Y, CHEN S M, CHEN Y, et al.In vitro induced tetraploid of Dendranthema nankingense (Nakai) Tzvel.Shows an improved level of abiotic stress tolerance[J].Scientia Horticulturae, 2011, 127:411-419.
    [16]
    LI W D, HU X, LIU J K, et al.Chromosome doubling can increase heat tolerance in Lonicera japonica as indicated by chlorophyⅡfluorescence imaging[J].BIOLOGIA PLANTARUM 55 (2) :279-284, 2011.
    [17]
    SINGH T N, AAPINALL D, PALAG L G.Proline aceumulation and varietal adaptability to drought in barley:A Potential metabolic meature of drought resistance[J].Nature New Biology, 1972, 236:188-190.
    [18]
    <image href="images/FJNX201405009_10800.jpg" display="" placement="break"></image>
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (201) PDF downloads(2) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return