Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus

FU Qiu-ling; FU Guang-hua; CHEN Hong-mei; SHI Shao-hua; CHEN Zhen; CHENG Long-fei; WAN Chun-he; LIN Jian-sheng; HUANG Yu

doi:10.19303/j.issn.1008-0384.2014.05.001

Volume 29 Issue 5

May 2014

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Article Navigation > Fujian Journal of Agricultural Sciences > 2014 > 29(5): 409-412

FU Qiu-ling, FU Guang-hua, CHEN Hong-mei, SHI Shao-hua, CHEN Zhen, CHENG Long-fei, WAN Chun-he, LIN Jian-sheng, HUANG Yu. Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 409-412. doi: 10.19303/j.issn.1008-0384.2014.05.001

Citation:

FU Qiu-ling, FU Guang-hua, CHEN Hong-mei, SHI Shao-hua, CHEN Zhen, CHENG Long-fei, WAN Chun-he, LIN Jian-sheng, HUANG Yu. Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 409-412. doi: 10.19303/j.issn.1008-0384.2014.05.001

Citation:

FU Qiu-ling, FU Guang-hua, CHEN Hong-mei, SHI Shao-hua, CHEN Zhen, CHENG Long-fei, WAN Chun-he, LIN Jian-sheng, HUANG Yu. Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus[J]. Fujian Journal of Agricultural Sciences, 2014, 29(5): 409-412. doi: 10.19303/j.issn.1008-0384.2014.05.001

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Cloning and Prokaryotic Expression of the VP1 Gene of Pancreotropic Duck Hepatitis Type 1 Virus

doi: 10.19303/j.issn.1008-0384.2014.05.001

Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences

Received Date: 2014-01-10
Publish Date: 2014-05-18

Abstract

Abstract

The structure protein (VP1) gene of pancreotropic duck hepatitis type 1virus MPZJ1206 strain was amplified by RT-PCR and integrated into the prokaryotic expression vector pGEX-6P-1, then expressed in E.coli.The SDS-PAGE results of the expressed protein demonstrated that the expression of recombinant VP1 protein of 52 kDa was in a high level, which laid a good foundation for the molecular diagnosis development and the function research of the VP1 protein.
- pancreotropic duck hepatitis type 1virus,
- VP1gene,
- prokaryotic expression

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References(21)

References

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